Dogan A, Başkaya M K, Rao V L, Rao A M, Dempsey R J
Department of Neurological Surgery, University of Wisconsin and Veterans Administration Hospital, Madison 53792, USA.
Neurol Res. 1998 Apr;20(3):265-70. doi: 10.1080/01616412.1998.11740517.
In models of middle cerebral artery occlusion using intraluminal suture, the size and the distribution of ischemic injury vary considerably among laboratories. In transcranial models of cerebral ischemia, a more consistent cerebral ischemic lesion is seen in Spontaneously Hypertensive rats (SHR). In the present study, we performed intraluminal suture occlusion of the MCA in SHR and compared its reproducibility with those in Sprague-Dawley (SD) rats. Male SHR and SD rats were anesthetized with halothane and subjected to 2 h of temporary middle cerebral artery occlusion by an intraluminal suture. Comparisons of regional cerebral blood flow figures taken throughout the experiment and lesion volume figures taken at 24 h after occlusion were made between the two groups. Total lesion volume in the SHR group was 263.6+/-30.5 mm3 (mean +/- SD), significantly larger and less variable than that in the SD group (145.4+/-123.7 mm3, p < 0.02). Throughout the ischemic period there was a tendency for rCBF to be lower in the SHR group than in the SD group. In some animals of both groups, recirculation could not be produced by withdrawal of the suture from the ICA. The mortality rate was 9% in the SD group and 17% in the SHR group. Intraluminal MCA occlusion in SHRs is associated with a more consistent, reliable and reproducible volume of ischemic injury. In the studies of temporary focal cerebral ischemia, this model must be accompanied by the monitoring of regional cerebral blood flow so as to obtain more reliable results.
在使用腔内缝合线的大脑中动脉闭塞模型中，各实验室之间缺血性损伤的大小和分布差异很大。在经颅脑缺血模型中，自发性高血压大鼠（SHR）出现的脑缺血损伤更为一致。在本研究中，我们对SHR进行了大脑中动脉腔内缝合闭塞，并将其可重复性与Sprague-Dawley（SD）大鼠的进行比较。雄性SHR和SD大鼠用氟烷麻醉，通过腔内缝合线进行2小时的大脑中动脉临时闭塞。对两组在整个实验过程中获取的局部脑血流量数据以及闭塞后24小时获取的损伤体积数据进行比较。SHR组的总损伤体积为263.6±30.5立方毫米（平均值±标准差），明显大于SD组（145.4±123.7立方毫米，p<0.02）且变异性更小。在整个缺血期，SHR组的局部脑血流量有低于SD组的趋势。在两组的一些动物中，通过从颈内动脉抽出缝合线无法实现再灌注。SD组的死亡率为9%，SHR组为17%。SHR的大脑中动脉腔内闭塞与更一致、可靠且可重复的缺血性损伤体积相关。在短暂性局灶性脑缺血研究中，该模型必须伴有局部脑血流量监测，以便获得更可靠的结果。
