含5-卤尿嘧啶的Z型DNA的光反应。

Photoreaction of 5-halouracil-containing Z-form DNA.

作者信息

Kawai K, Sugiyama H, Saito I

机构信息

Department of Synthetic Chemistry and Biological Chemistry, Faculty of Engineering, Kyoto University, Japan.

出版信息

Nucleic Acids Symp Ser. 1997(37):93-4.

PMID:9586015

Abstract

Photoreactions of IU-containing Z-form DNA were investigated. By introducing 8-methylguanine, stable Z-form IU-containing oligonucleotides were prepared. DNA conformational change from B to Z leads to the remarkable acceleration of the photoreactivity of IU-containing oligonucleotides. In particular 2'-hydrogen abstraction was observed preferentially in Z-form DNA, and 2'-hydroxylation occurred specifically at the 5' side of IU in Z-form DNA.

摘要

对含碘尿嘧啶（IU）的Z型DNA的光反应进行了研究。通过引入8-甲基鸟嘌呤，制备了稳定的含IU的Z型寡核苷酸。DNA从B型到Z型的构象变化导致含IU的寡核苷酸光反应性显著加速。特别是在Z型DNA中优先观察到2'-氢的提取，并且在Z型DNA中IU的5'侧特异性地发生2'-羟基化。