DNA-壳聚糖柱的制备及其应用：致癌物与柱的结合

Preparation of DNA-chitosan columns and their applications: binding of carcinogens to the column.

作者信息

Hayatsu H, Tanaka Y, Negishi K

机构信息

Faculty of Pharmaceutical Sciences, Okayama University, Japan.

出版信息

Nucleic Acids Symp Ser. 1997(37):139-40.

PMID:9586038

Abstract

DNA, either native or denatured, can be immobilized by complexing with chitosan. The DNA-chitosan, which is insoluble in aqueous media, contains DNA at about 50% in weight. The DNA in the complex is accessible by enzymes and reagents. Carcinogenic heterocyclic amines, having affinity to DNA, can be adsorbed to the DNA-chitosan. The DNA-chitosan complex can be homogeneously dispersed in celite powder, and the resultant DNA-chitosan-celite is a material suitable to construct an immobilized DNA-column. The column was shown to be effective in concentrating heterocyclic amines.

摘要

天然或变性的DNA都可以通过与壳聚糖复合来固定。不溶于水介质的DNA-壳聚糖含有约50%（重量）的DNA。复合物中的DNA可被酶和试剂接触到。对DNA有亲和力的致癌杂环胺可以吸附到DNA-壳聚糖上。DNA-壳聚糖复合物可以均匀地分散在硅藻土粉末中，所得的DNA-壳聚糖-硅藻土是一种适合构建固定化DNA柱的材料。该柱被证明在浓缩杂环胺方面是有效的。