Sandborgh-Englund G, Björkman L, Björkman L, Valtersson C
Department of Basic Oral Science, Karolinska Institute, Huddinge Hospital, Sweden.
Scand J Clin Lab Invest. 1998 Apr;58(2):155-60. doi: 10.1080/00365519850186742.
A sensitive and semi-automated analytical method allowing determination of low and normal levels of total mercury in human blood and plasma using cold vapour atomic fluorescence is described. Samples are digested overnight, or at an elevated temperature for 4 h, followed by bromination at room temperature. After reduction with tin (II), analysis is performed using automated continuous flow vapour generation coupled to a fluorescence detector, allowing 20 samples to be analysed per hour. Detection limits for blood and plasma were found to be 0.9 and 0.5 nmol Hg l-1, respectively. The method precision at various concentrations of mercury was determined. For whole blood at 8.1 nmol Hg l-1 and 12.9 nmol Hg l-1, the within-day precision was 5% and 6% and the between-day precision 9% and 6%, respectively. For plasma at 1.3 nmol Hg l-1, the within-day precision was 13% while the between-day precision was 17%. Accuracy was evaluated by an inter-laboratory comparison study. At blood mercury concentrations below 60 nmol Hg l-1 the results from the current method were almost identical to those obtained with radiochemical neutron activation analysis, commonly regarded as a reference method. The present method should have merits in relation to previously used methods using atomic absorption spectrometry.
本文描述了一种灵敏的半自动分析方法，该方法采用冷蒸气原子荧光法测定人体血液和血浆中低水平和正常水平的总汞。样品过夜消化，或在高温下消化4小时，然后在室温下进行溴化处理。用锡（II）还原后，使用自动连续流动蒸气发生装置与荧光检测器联用进行分析，每小时可分析20个样品。血液和血浆的检测限分别为0.9和0.5 nmol Hg l-1。测定了不同汞浓度下该方法的精密度。对于全血中8.1 nmol Hg l-1和12.9 nmol Hg l-1的情况，日内精密度分别为5%和6%，日间精密度分别为9%和6%。对于血浆中1.3 nmol Hg l-1的情况，日内精密度为13%，日间精密度为17%。通过实验室间比较研究评估了准确性。在血液汞浓度低于60 nmol Hg l-1时，本方法的结果与通常被视为参考方法的放射化学中子活化分析所获得的结果几乎相同。与先前使用的原子吸收光谱法相比，本方法应具有优点。
