Dada A J, Ajayi A O, Diamondstone L, Quinn T C, Blattner W A, Biggar R J
Viral Epidemiology Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20852, USA.
Sex Transm Dis. 1998 May;25(5):237-42. doi: 10.1097/00007435-199805000-00004.
To determine the prevalence rates of serological reactivity of Haemophilus (H.) ducreyi, Treponema pallidum, and herpes simplex virus type 2 (HSV-2) antibodies among female sex workers (FSWs) and their association with human immunodeficiency virus (HIV) antibody status.
Cross-sectional, standard serological assays were used for syphilis, HSV-2 and HIV; a modified enzyme-linked immunosorbent assay (ELISA) was used to detect specific anti-H. ducreyi immunoglobulin (Ig) G and IgA antibodies.
Seroprevalence rates were 86% for anti-H. ducreyi IgG and 69% for anti-H. ducreyi IgA; 4% for rapid plasma reagin (RPR) and Treponema palladium hemagglutination assay (TPHA) confirmed syphilis; 59% for HSV-2; 12% for HIV-1 and 2% for HIV-2. Lower-class FSWs were significantly more likely than upper-class FSWs to be H. ducreyi seropositive (IgG: OR = 42.7; IgA: OR = 7.6) and have current or past syphilis infection (RPR: OR = 3.5; RPR and TPHA: OR = 4.5). The presence of syphilis increased significantly with older age (P-trend < 0.001). Non-Nigerian FSWs had significantly higher reactivity to chancroid (IgG: OR = 3.5; IgA: OR = 1.8) and borderline reactivity to syphilis (RPR: OR = 1.6; TPHA: OR = 2.0). A history of sex with non-Nigerian Africans was significantly associated with chancroid reactivity and borderline significant with syphilis serostatus. H. ducreyi seropositivity was significantly more likely in FSWs with HSV-2 (OR = 2.4) and syphilis (OR = 5.6). Chancroid and HSV-2 antibodies were also more common in HIV-infected FSWs.
The prevalence of H. ducreyi antibodies is the highest rate that has been reported. Our findings underscore the importance of an effective program to control GUDs as part of the strategy to prevent the potentially explosive spread of HIV in Nigeria.
确定女性性工作者中杜克雷嗜血杆菌、梅毒螺旋体及2型单纯疱疹病毒（HSV-2）抗体的血清学反应患病率及其与人类免疫缺陷病毒（HIV）抗体状态的关联。
采用横断面研究，梅毒、HSV-2和HIV检测使用标准血清学检测方法；改良酶联免疫吸附试验（ELISA）用于检测特异性抗杜克雷嗜血杆菌免疫球蛋白（Ig）G和IgA抗体。
抗杜克雷嗜血杆菌IgG血清阳性率为86%，抗杜克雷嗜血杆菌IgA血清阳性率为69%；快速血浆反应素环状卡片试验（RPR）和梅毒螺旋体血凝试验（TPHA）确诊梅毒的患病率为4%；HSV-2患病率为59%；HIV-1患病率为12%，HIV-2患病率为2%。下层女性性工作者杜克雷嗜血杆菌血清阳性（IgG：比值比[OR]=42.7；IgA：OR=7.6）及现患或曾患梅毒感染（RPR：OR=3.5；RPR和TPHA：OR=4.5）的可能性显著高于上层女性性工作者。梅毒患病率随年龄增长显著升高（P趋势<0.001）。非尼日利亚女性性工作者软下疳反应性显著更高（IgG：OR=3.5；IgA：OR=1.8），梅毒临界反应性更高（RPR：OR=1.6；TPHA：OR=2.）。与非尼日利亚非洲人有性行为史与软下疳反应性显著相关，与梅毒血清状态临界显著相关。感染HSV-2（OR=2.4）和梅毒（OR=5.6）的女性性工作者杜克雷嗜血杆菌血清阳性的可能性显著更高。软下疳和HSV-2抗体在感染HIV的女性性工作者中也更常见。
杜克雷嗜血杆菌抗体患病率为已报道最高。我们的研究结果强调了实施有效方案控制生殖器溃疡疾病作为尼日利亚预防HIV潜在爆发性传播策略一部分的重要性。
