Beltes P, Koulaouzidou E, Kolokuris I, Kortsaris A H
Department of Dental Pathology and Therapeutics, Faculty of Dentistry, Aristotelion University of Thessaloniki, Greece.
J Endod. 1997 Sep;23(9):572-4. doi: 10.1016/S0099-2399(06)81124-6.
The cytotoxicity of two glass-ionomer root canal sealers (Ketac-Endo and Endion) was tested by using an established cell line, BHK21/C13. Under aseptic conditions, the sealers were prepared according to the manufacturers' directions, and 0.1 ml of each material was placed in petri dishes. After setting for 6 h, the sealers were covered with 20 x 10(4) cells per dish. The cultures were incubated at 37 degrees C for 24 h, 48 h, and 72 h. Cytotoxicity was assessed by a quantitative technique at three observation periods. Endion was highly cytotoxic, causing a significant decrease in cell density. Ketac-Endo proved to be a very biocompatible material.
使用已建立的细胞系BHK21/C13测试了两种玻璃离子根管封闭剂（Ketac-Endo和Endion）的细胞毒性。在无菌条件下，按照制造商的说明制备封闭剂，每种材料取0.1 ml置于培养皿中。凝固6小时后，每个培养皿覆盖2×10⁴个细胞。将培养物在37℃下孵育24小时、48小时和72小时。在三个观察期通过定量技术评估细胞毒性。Endion具有高细胞毒性，导致细胞密度显著降低。Ketac-Endo被证明是一种生物相容性非常好的材料。
