Biomedical Science Team, Chang Gung Institute of Technology, Taoyuan, Taiwan.
Int Endod J. 2010 Mar;43(3):251-7. doi: 10.1111/j.1365-2591.2009.01676.x.
To evaluate the cytotoxicity of current root canal sealers to periodontal ligament (PDL) fibroblasts.
Five root canal sealers (Canals, Canals-N, Topseal, Sealapex, Tubliseal) were prepared and placed into transwells. After initial setting for 1 h, the transwells with sealers were placed into cultured PDL fibroblasts. They were cultured for further 3 or 18 h. Morphological changes were observed. Cell viability was estimated by 3-(4,5-dimethyl-thiazol-2-yl)-2,5- diphenyl-tetrazolium bromide (MTT) assay.
Marked retraction and death of PDL fibroblasts were observed after exposure to Canals or Topseal for 3 h. A 3-h exposure of PDL fibroblasts to Tubliseal stimulated MTT reduction. Canals-N showed little cytotoxicity even after an exposure of 18 h.
Canals was the most toxic sealer, followed by Topseal. Sealapex and Tubliseal had comparable and moderate cytotoxicity to PDL fibroblasts, whereas Canals-N showed little cytotoxicity. Exposure to Tubliseal may modulate MTT reduction in PDL fibroblasts. Canals-N had good biocompatibility.
评价目前根管封闭剂对牙周膜（PDL）成纤维细胞的细胞毒性。
制备了 5 种根管封闭剂（Canals、Canals-N、Topseal、Sealapex、Tubliseal）并放入 Transwell 中。初始凝固 1 小时后，将含封闭剂的 Transwell 放入培养的 PDL 成纤维细胞中。培养 3 或 18 小时后，观察形态变化。通过 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）测定法评估细胞活力。
暴露于 Canals 或 Topseal 3 小时后，PDL 成纤维细胞明显回缩和死亡。Tubliseal 暴露 3 小时可刺激 MTT 减少。即使暴露 18 小时，Canals-N 也表现出很小的细胞毒性。
Canals 是最具毒性的封闭剂，其次是 Topseal。Sealapex 和 Tubliseal 对 PDL 成纤维细胞具有相似且中等的细胞毒性，而 Canals-N 则表现出很小的细胞毒性。Tubliseal 的暴露可能会调节 PDL 成纤维细胞中 MTT 的减少。Canals-N 具有良好的生物相容性。
