使用细胞培养技术评估根分叉穿孔修复材料的生物相容性：Ketac Molar与ProRoot MTA的对比
Biocompatibility of furcal perforation repair material using cell culture technique: Ketac Molar versus ProRoot MTA.
作者信息
Vajrabhaya La-ongthong, Korsuwannawong Suwanna, Jantarat Jeeraphat, Korre Sumeth
机构信息
Department of Operative Dentistry, Faculty of Dentistry, Mahidol University, Bangkok, Thailand.
出版信息
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2006 Dec;102(6):e48-50. doi: 10.1016/j.tripleo.2006.05.015. Epub 2006 Sep 26.
OBJECTIVE
The objective of this study was to evaluate the cytotoxicity of furcal perforation repair materials, GI and MTA, using cell culture technique.
STUDY DESIGN
The extract of ProRoot MTA and Ketac Molar were treated on PDL cells in a 96-well tissue-culture plate. Cell proliferation after an incubation period of 3 days was determined by using MTT assay.
RESULTS
The growth of cultured human periodontal fibroblast cells were suppressed by both perforation repair materials. The percent of cell viability in the Ketac Molar group was lower than in the ProRoot MTA group (P = .000).
CONCLUSIONS
Although Ketac Molar has the advantage of adhering to dentine, it is more cytotoxic to the PDL cells than MTA. In selecting the perforation repair material, it is recommended not only to consider the sealing ability of the material with dentine but also the biocompatibility of material to the underlying tissue.
目的
本研究的目的是使用细胞培养技术评估用于根分叉穿孔修复的材料GI和MTA的细胞毒性。
研究设计
将ProRoot MTA和Ketac Molar的提取物在96孔组织培养板中处理人牙周膜细胞。通过MTT法测定培养3天后的细胞增殖情况。
结果
两种穿孔修复材料均抑制了培养的人牙周膜成纤维细胞的生长。Ketac Molar组的细胞活力百分比低于ProRoot MTA组（P = 0.000）。
结论
尽管Ketac Molar具有粘附于牙本质的优点，但它对牙周膜细胞的细胞毒性比MTA更大。在选择穿孔修复材料时，建议不仅要考虑材料与牙本质的封闭能力，还要考虑材料对下方组织的生物相容性。
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