Maunoury M R
C R Acad Hebd Seances Acad Sci D. 1978 Feb 13;286(6):503-6.
Sera of 40 normal nonimmunized rabbits were eprouved by immunoperoxidase on cultural human cells. Four sera contain centrosphere-reactive antibodies and for the strongest serum the centrospheres were still stained with the 1/600 dilution. This last serum visualized the centrospheres within the height human cell lines eprouved, whatever the phase of the mitotic cycle. The staining was not inhibited after serum absorption by tubulin.
用免疫过氧化物酶法在培养的人类细胞上检测了40只正常未免疫兔子的血清。四份血清含有中心球反应性抗体,对于最强的那份血清,中心球在1/600稀释度时仍能被染色。在检测的所有人类细胞系中,无论处于有丝分裂周期的哪个阶段,这最后一份血清都能使中心球显色。用微管蛋白吸收血清后,染色并未受到抑制。