小牛胸腺中心体的大规模分离：一种特定构型的鉴定
Mass isolation of calf thymus centrosomes: identification of a specific configuration.
作者信息
Komesli S, Tournier F, Paintrand M, Margolis R L, Job D, Bornens M
机构信息
Laboratoire Biochimie des Régulations Cellulaires Endocrines, Institut National de la Santé et de la Recherche Médicale U244, Grenoble, France.
出版信息
J Cell Biol. 1989 Dec;109(6 Pt 1):2869-78. doi: 10.1083/jcb.109.6.2869.
Abstract
Centrosomes from calf thymocytes were isolated using a simple preparative procedure that provides large yields of free organelles. A comparative study with centrosomes isolated from human cultured lymphoblasts has led to the discovery of important differences in the structure of the two isolates and in their capacity to nucleate microtubules from purified tubulin. The possibility that the centrosomal structure depends upon the growth state of cells is discussed.
摘要
利用一种简单的制备方法从小牛胸腺细胞中分离出中心体，该方法可大量获取游离细胞器。一项对从人类培养淋巴细胞中分离出的中心体进行的比较研究，发现了这两种分离物在结构以及从纯化微管蛋白中形成微管的能力方面存在重要差异。文中还讨论了中心体结构是否取决于细胞生长状态的可能性。
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Mass isolation of calf thymus centrosomes: identification of a specific configuration.小牛胸腺中心体的大规模分离：一种特定构型的鉴定
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