Davis W C, Zuckermann F A, Hamilton M J, Barbosa J I, Saalmüller A, Binns R M, Licence S T
Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman 99164-7040, USA.
Vet Immunol Immunopathol. 1998 Jan 30;60(3-4):305-16. doi: 10.1016/s0165-2427(97)00107-4.
Thirty two monoclonal antibodies (mAbs) from the first round of analysis in the Second International Swine CD Workshop were placed together with additional mAb derived from the first workshop in the null cell panel for further evaluation. Preparations of peripheral blood leukocytes, concanavalin A stimulated peripheral blood mononuclear cells, and spleen cells were used in flow cytometric analyses. Nineteen mAbs identified molecules that were not expressed on null cells, not lineage specific, or recognized activation molecules. Sixteen mAbs including control mAbs were identified that were specific for null cells. One of the latter mAbs, 041 (PGBL22A), that recognizes a determinant on a constant region of porcine gamma delta TcR established the majority of null cells are gamma delta T cells. Use of this mAb in further comparisons demonstrated the gamma delta T cell population is comprised of two major subpopulations, one negative and one positive for CD2. Two color analyses demonstrated that 11 of the mAbs formed a broad cluster that included control mAbs 188 (MAC320) that defined the CD2 negative SWC6 cluster in the first workshop and mAb 122 (CC101) that might recognize an orthologue of bovine WC1 and nine mAbs that recognize determinants on one or more molecules with overlapping patterns of expression on subsets of CD2- gamma delta T cells. Two groups of mAbs formed the previously identified subset clusters SWC4 and SWC5. Two new mAbs formed a third subcluster. Three mAbs did not form clusters. Three mAbs predicted to recognize TcR in the first workshop (020 [PT14A], 021 [PT79A], and 022 [MUC127A]) and mAb PGBL22A were shown to immunoprecipitate a 37, 40 kDa heterodimer.
在第二届国际猪CD研讨会上第一轮分析得到的32种单克隆抗体（mAb）与来自第一届研讨会的其他单克隆抗体一起被置于空细胞组中进行进一步评估。外周血白细胞、伴刀豆球蛋白A刺激的外周血单个核细胞和脾细胞制剂用于流式细胞术分析。19种单克隆抗体识别的分子在空细胞上不表达、不是谱系特异性的或识别激活分子。包括对照单克隆抗体在内的16种单克隆抗体被鉴定为空细胞特异性的。后一种单克隆抗体中的一种，即识别猪γδTcR恒定区上一个决定簇的041（PGBL22A），确定了大多数空细胞是γδT细胞。在进一步比较中使用这种单克隆抗体表明，γδT细胞群体由两个主要亚群组成，一个CD2阴性，一个CD2阳性。双色分析表明，11种单克隆抗体形成了一个广泛的簇，其中包括在第一届研讨会上定义CD2阴性SWC6簇的对照单克隆抗体188（MAC320）和可能识别牛WC1直系同源物的单克隆抗体122（CC101），以及9种识别CD2 - γδT细胞亚群上具有重叠表达模式的一个或多个分子上决定簇的单克隆抗体。两组单克隆抗体形成了先前鉴定的亚群簇SWC4和SWC5。两种新的单克隆抗体形成了第三个亚簇。三种单克隆抗体未形成簇。在第一届研讨会上预测识别TcR的三种单克隆抗体（020 [PT14A]、021 [PT79A]和022 [MUC127A]）以及单克隆抗体PGBL22A被证明能免疫沉淀一种37、40 kDa的异二聚体。
