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与猪CD6反应的单克隆抗体分析

Analyses of monoclonal antibodies reactive with porcine CD6.

作者信息

Saalmüller A, Aasted B, Canals A, Dominguez J, Goldman T, Lunney J K, Maurer S, Pauly T, Pescovitz M D, Pospisil R

机构信息

Federal Research Centre for Virus Diseases of Animals, Tübingen, Germany.

出版信息

Vet Immunol Immunopathol. 1994 Oct;43(1-3):243-7. doi: 10.1016/0165-2427(94)90143-0.

DOI:10.1016/0165-2427(94)90143-0
PMID:7531907
Abstract

Amongst the monoclonal antibodies (mAbs) submitted to the first porcine CD workshop, two mAbs (workshop numbers 055 and 120) could be identified to recognize the porcine CD6 analogue. Both mAbs seemed to be highly T-cell specific and showed neither reactivity with cells of the myeloic lineage nor with B lymphocytes. The observed molecular mass of the antigen precipitated by mAb 120 of 110 kDa confirmed this classification. Without molecular analyses of the antigen recognized by mAb 055, but similar staining pattern in FCM compared with 120, mAb 055 was allocated to the wCD6 subcluster.

摘要

在提交给首次猪CD研讨会的单克隆抗体(mAb)中,可鉴定出两种mAb(研讨会编号055和120)识别猪CD6类似物。两种mAb似乎都具有高度的T细胞特异性,对髓系谱系细胞和B淋巴细胞均无反应性。mAb 120沉淀的抗原观察到的分子量为110 kDa,证实了这种分类。虽然没有对mAb 055识别的抗原进行分子分析,但与120相比,在流式细胞术中具有相似的染色模式,mAb 055被归入wCD6亚群。

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