Licence S T, Davis W C, Carr M M, Binns R M
Immunology Department, Babraham Institute, Cambridge, UK.
Vet Immunol Immunopathol. 1995 Aug;47(3-4):253-71. doi: 10.1016/0165-2427(95)05444-b.
Further studies were carried out on the monoclonal antibodies (mAbs) from the First International Swine CD Workshop which react with gamma delta Null T-lymphocytes, defined by the binding of mAb w020/141 (MAC320) as Swine Workshop Cluster number SWC6. Studies were also carried out on several other mAbs from the same workshop which identify other CD antigens, but whose binding is not restricted exclusively to gamma delta Null T-lymphocytes. The first group consists of 11 mAbs (w021, w022, w059-w065, w105 and w117) and the second group of 18 mAbs (w008, w026, w056, w067-w071, w080, w091-w094, w110, w111, w118, w119 and w121). All mAbs were characterised by binding to peripheral blood lymphocytes (PBL) from normal, sham-thymectomized (STx) and thymectomized (Tx) pigs of the Babraham SLAb/b line and by their overlap, using two-colour immunofluorescence with biotinylated mAb MAC320 (w020/141), which identifies all gamma delta Null cell T-lymphocytes and the Null cell subpopulation identified by MAC319 (w021). The Null cell-specific mAbs were also used in inhibition studies of MAC319 and MAC320 binding and by staining PBL with pairs of mAbs together with either MAC319 or MAC320. Based on these data we suggest a putative relationship of the Null cell subsets defined by these mAbs with each other.
对来自第一届国际猪CD研讨会的单克隆抗体（mAb）进行了进一步研究，这些单克隆抗体与γδ无T淋巴细胞反应，通过单克隆抗体w020/141（MAC320）的结合定义为猪研讨会集群编号SWC6。还对来自同一研讨会的其他几种单克隆抗体进行了研究，这些抗体识别其他CD抗原，但其结合并不局限于γδ无T淋巴细胞。第一组由11种单克隆抗体（w021、w022、w059 - w065、w105和w117）组成，第二组由18种单克隆抗体（w008、w026、w056、w067 - w071、w080、w091 - w094、w110、w111、w118、w119和w121）组成。所有单克隆抗体的特征在于与来自Babraham SLAb/b系正常、假胸腺切除（STx）和胸腺切除（Tx）猪的外周血淋巴细胞（PBL）结合，并通过双色免疫荧光与生物素化单克隆抗体MAC320（w020/141）重叠，MAC320可识别所有γδ无细胞T淋巴细胞以及由MAC319（w021）识别的无细胞亚群。无细胞特异性单克隆抗体还用于MAC319和MAC320结合的抑制研究，并通过将单克隆抗体对与MAC319或MAC320一起对PBL进行染色。基于这些数据，我们提出了由这些单克隆抗体定义的无细胞亚群之间的假定关系。
