Takahashi Y, Ellis L M, Ohta T, Mai M
Department of Surgery, Cancer Research Institute, Kanazawa University, Japan.
Surg Today. 1998;28(4):367-72. doi: 10.1007/s005950050143.
We studied the role of angiogenesis in patients with medullary type poorly differentiated adenocarcinoma (MTPDA) of the stomach. Immunohistochemical analyses were conducted using antibodies against factor VIII (endothelial cells), vascular endothelial growth factor (VEGF) and its receptors (KDR andflt-1), and basic fibroblast growth factor (bFGF) and its receptors (bek andflg). Archival specimens of MTPDA (n=22) and non-MTPDA (n=47) were studied. The expression of VEGF and bFGF, the vessel count, and positivity of KDR on endothelium were all significantly higher in MTPDA than in non-MTPDA. The vessel count correlated with the VEGF expression in MTPDA. The vessel count and VEGF expression increased with the increasing stage of disease in MTPDA but not in non-MTPDA. The expression of bFGF and its receptors did not correlate with the vessel count and stage of disease in either type. These findings thus suggest that the biological behavior of medullary type poorly differentiated adenocarcinoma of the stomach is angiogenesis-dependent. The correlation of the VEGF expression and its endothelial receptors with the vessel count and the stage of disease thus suggests that VEGF is a factor responsible for the induction of angiogenesis in this type.
我们研究了血管生成在胃髓样型低分化腺癌（MTPDA）患者中的作用。使用抗因子VIII（内皮细胞）、血管内皮生长因子（VEGF）及其受体（KDR和flt-1）、碱性成纤维细胞生长因子（bFGF）及其受体（bek和flg）的抗体进行免疫组织化学分析。对MTPDA存档标本（n = 22）和非MTPDA存档标本（n = 47）进行了研究。MTPDA中VEGF和bFGF的表达、血管计数以及内皮细胞上KDR的阳性率均显著高于非MTPDA。MTPDA中的血管计数与VEGF表达相关。MTPDA中血管计数和VEGF表达随疾病分期增加而增加，但非MTPDA中并非如此。bFGF及其受体的表达在两种类型中均与血管计数和疾病分期无关。因此，这些发现表明胃髓样型低分化腺癌的生物学行为依赖于血管生成。VEGF表达及其内皮受体与血管计数和疾病分期的相关性表明，VEGF是该类型中诱导血管生成的一个因素。
