Zhang Z, Lu S, Wang J
Dept. of Orthopaedic Surgery, General Hospital of Chinese PLA, Beijing.
Zhonghua Wai Ke Za Zhi. 1996 Oct;34(10):596-8.
BMP is one of important factors in the pathophysiology of bone regeneration. Fifteen New Zealand rabbits were used in this experiment. We studied the distribution and effectiveness of endogenic BMP on a 10 mm bone defect of radius, by utilizing immunohistochemistry of BMP and quantitative computer imaging system. On the 3rd day, death of osteocytes and BMP positive blood clot were observed. The mesenchymal cells from periosteum and endoosteum, and osteoblast were also BMP positive. By quantitative study, we found there was a gradient distribution of BMP in bone defect, i.e, the value of BMP decreased gradually along the distance from the fracture ends. The maximal value of BMP was noted at the 1st week postoperation. In conclusion, two sources of endogenic BMP were found, one was from the absorption of necrotic tissue of fracture ends, the other was from the secretion of osteogenic mesenchymal cells during the process of bone regeneration. Nonunion of bone defect was caused in part by the gradient distribution of BMP. Accordingly, the concept of effective quantity of endogenic BMP was drawn rosen. It might be a new method in the treatment of bone defect by increasing the concentration of endogenic BMP and improving its distribution.
骨形态发生蛋白（BMP）是骨再生病理生理学中的重要因素之一。本实验采用15只新西兰兔。我们通过BMP免疫组织化学和定量计算机成像系统，研究内源性BMP在兔桡骨10mm骨缺损处的分布及作用效果。术后第3天，观察到骨细胞死亡及BMP阳性血凝块。骨膜和骨内膜的间充质细胞以及成骨细胞也呈BMP阳性。通过定量研究，我们发现骨缺损处BMP呈梯度分布，即BMP值沿距骨折端的距离逐渐降低。术后第1周BMP值最高。总之，发现内源性BMP有两个来源，一个是骨折端坏死组织的吸收，另一个是骨再生过程中成骨间充质细胞的分泌。骨缺损不愈合部分是由BMP的梯度分布所致。据此，提出了内源性BMP有效量的概念。通过提高内源性BMP的浓度并改善其分布，可能是治疗骨缺损的一种新方法。
