Sun J, Ito T, Zhang P
Department of Surgery, Beijing Hospital.
Zhonghua Wai Ke Za Zhi. 1996 Nov;34(11):685-7.
In this study, 18 BUF rats with 7316 A liver cancer burden were divided into three groups. Groups A underwent simple laparotomy, group B had 70% hepatectomy, and group C with laparotomy plus TGF-beta . Splenic adhesive cells and serum of postoperative day 5 in both group A and B were added into mixed lymphocellular culture and CCL-64 cell culture, TGF-beta was also added into MLC and 7613 A liver cancer culture. It was found that in group B and C the growth rate of tumor cells was greatly accelerated (P < 0.01); whereas MLC and CCL-64 cell proliferation was inhibited by the serum and splenic adhesive cells from group B (P < 0.05). TGF-beta also significantly inhibited MLC through it had no effect on 7316 A liver cancer cells. The authors came to the conclusion that there was the activity of TGF-beta in the serum of the rats with partial hepatectomy which inhibits the proliferation of host immune cells.
在本研究中，将18只荷7316A肝癌的BUF大鼠分为三组。A组行单纯剖腹术，B组行70%肝切除术，C组行剖腹术加转化生长因子-β（TGF-β）。将A组和B组术后第5天的脾黏附细胞和血清加入混合淋巴细胞培养和CCL-64细胞培养中，TGF-β也加入混合淋巴细胞培养和7613A肝癌培养中。结果发现，B组和C组肿瘤细胞的生长速度明显加快（P<0.01）；而B组的血清和脾黏附细胞抑制了混合淋巴细胞培养和CCL-64细胞的增殖（P<0.05）。TGF-β也显著抑制混合淋巴细胞培养，尽管它对7316A肝癌细胞没有影响。作者得出结论，部分肝切除大鼠血清中存在TGF-β活性，其抑制宿主免疫细胞的增殖。
