Guichard C, Gilain L, Abd-Al Samad I, Piron G, Brugel L, Escudier E, Coste A
Department of Otorhinolaryngology and Head and Neck Surgery, Gabriel Montpied University Hospital, Faculty of Medicine, University of Clermont-Ferrand, France.
Laryngoscope. 1998 May;108(5):716-20. doi: 10.1097/00005537-199805000-00017.
Inverted papillomas (IPs) are rare benign tumors of nasal epithelium with high recurrence rates and malignant transformation potential. Their etiology is still uncertain, and the mechanism of their growth has not yet been fully described. The purpose of this study was to detect, quantify, and compare cell proliferation, apoptosis, and apoptosis inhibition in hyperplastic epithelium from IPs and in inflammatory nasal polyps (NPs). IP samples were obtained after surgical removal of tumor in 13 patients, and NPs were sampled during endoscopic ethmoidectomy in 10 patients with nasal polyposis. Cell proliferation and apoptosis inhibition, respectively, were assessed by immunohistochemical identification of the proliferating cell nuclear antigen (PCNA) and the oncoprotein Bcl-2. Apoptosis was evaluated by analyzing the DNA fragmentation. Cell proliferation and apoptosis were significantly higher in IPs than in NPs (P = .0002 and P = .043, respectively), while apoptosis inhibition was significantly lower in IPs than in NPs (P = .001). Concerning IPs, cell proliferation was significantly higher than apoptosis (P = .0029) and apoptosis inhibition (P = .0015). The increase in epithelial cell proliferation seemed to be greater in IPs with dysplasia than in IPs without dysplasia. Increased epithelial cell proliferation, but not apoptosis and apoptosis inhibition, seems to be involved in the development of IP.
内翻性乳头状瘤（IPs）是鼻腔上皮的罕见良性肿瘤，具有高复发率和恶变潜能。其病因仍不确定，生长机制尚未完全阐明。本研究旨在检测、量化并比较IPs增生上皮及炎性鼻息肉（NPs）中的细胞增殖、凋亡及凋亡抑制情况。13例患者手术切除肿瘤后获取IP样本，10例鼻息肉病患者在内镜筛窦切除术中采集NPs样本。分别通过增殖细胞核抗原（PCNA）和癌蛋白Bcl-2的免疫组化鉴定评估细胞增殖和凋亡抑制情况。通过分析DNA片段化评估凋亡情况。IPs中的细胞增殖和凋亡显著高于NPs（分别为P = 0.0002和P = 0.043），而IPs中的凋亡抑制显著低于NPs（P = 0.001）。关于IPs，细胞增殖显著高于凋亡（P = 0.0029）和凋亡抑制（P = 0.0015）。发育异常的IPs中上皮细胞增殖的增加似乎比无发育异常的IPs更大。上皮细胞增殖增加而非凋亡及凋亡抑制似乎参与了IP的发生发展。
