[口腔鳞状细胞癌中人乳头瘤病毒的研究]

[Study of HPV in oral squamous cell carcinoma].

作者信息

Lei L, Li H, Sun Y

机构信息

Department of Stomatology, Medical College, Jinan University, Guangzhou.

出版信息

Zhonghua Kou Qiang Yi Xue Za Zhi. 1996 Nov;31(6):375-7.

PMID:9592298

Abstract

In order to demonstrate the presence of human papillomavirus (HPV) in oral squamous cell carcinoma (OSCC), we detected HPV 16 and 18 type DNA in OSCC and normal mucosa (NOM) by polymerase chain reaction (PCR), then analysed the PCR products using southern blot hybridization. The study shows that the positive rates of HPV DNA were 47.8% (11/23) in OSCC, including HPV16 in 6 samples, HPV18 in 3 samples and 16, 18 co-infection in 2 samples. In contrast, HPV16 DNA were found to be 20% (2/10) in NOM. The results suggest that HPV may play a role in carcinogenesis of OSCC.

摘要

为了证明口腔鳞状细胞癌（OSCC）中存在人乳头瘤病毒（HPV），我们通过聚合酶链反应（PCR）检测了OSCC和正常黏膜（NOM）中的HPV 16和18型DNA，然后使用Southern印迹杂交分析PCR产物。研究表明，OSCC中HPV DNA的阳性率为47.8%（11/23），其中6份样本为HPV16，3份样本为HPV18，2份样本为16、18型共同感染。相比之下，NOM中HPV16 DNA的阳性率为20%（2/10）。结果表明，HPV可能在OSCC的致癌过程中发挥作用。