在正常、发育异常及恶性口腔上皮中检测人乳头瘤病毒16型和18型DNA

Detection of human papillomavirus-16 and HPV-18 DNA in normal, dysplastic, and malignant oral epithelium.

作者信息

Sugiyama Masaru, Bhawal Ujjal Kumar, Dohmen Tamiko, Ono Shigehiro, Miyauchi Miwa, Ishikawa Takenori

机构信息

Department of Oral and Maxillofacial Surgery, Graduate School of Biomedical Sciences, Hiroshima University, Kasumi 1-2-3, Minami-Ku, Hiroshima 734-8553, Japan.

出版信息

Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2003 May;95(5):594-600. doi: 10.1067/moe.2003.36.

DOI:10.1067/moe.2003.36

PMID:12738951

Abstract

OBJECTIVE

Our aim was to clarify the association of human papillomavirus (HPV) with oral carcinogenesis, especially its early stage.

STUDY DESIGN

Tissue specimens of normal mucosa, epithelial dysplasia, oral squamous cell carcinoma (OSCC), and OSCC cell lines were examined for the presence of HPV-16 and HPV-18 E6 DNA by means of the polymerase chain reaction test.

RESULTS

The detection rate of HPV-16 in epithelial dysplasia (31/51) was higher than that in normal mucosa (16/44) and in OSCC (30/86) and was statistically different from that in OSCC. The cases that progressed from epithelial dysplasia to carcinoma showed a significantly higher HPV-16 detection rate than the other cases in both epithelial dysplasia and OSCC. HPV-16 and HPV-18 were detected only at early passages of 2 of 10 OSCC cell lines.

CONCLUSIONS

These results strongly suggest that HPV-16 may be involved in the early stages of the development of some oral carcinomas.

摘要

目的

我们的目的是阐明人乳头瘤病毒（HPV）与口腔癌发生的关联，尤其是其早期阶段。

研究设计

通过聚合酶链反应检测，对正常黏膜、上皮发育异常、口腔鳞状细胞癌（OSCC）及OSCC细胞系的组织标本进行HPV - 16和HPV - 18 E6 DNA检测。

结果

上皮发育异常中HPV - 16的检出率（31/51）高于正常黏膜（16/44）和OSCC（30/86），且与OSCC中的检出率有统计学差异。从上皮发育异常进展为癌的病例，在上皮发育异常和OSCC中，HPV - 16检出率均显著高于其他病例。在10个OSCC细胞系中，仅2个细胞系的早期传代中检测到HPV - 16和HPV - 18。