Sugiyama Masaru, Bhawal Ujjal Kumar, Dohmen Tamiko, Ono Shigehiro, Miyauchi Miwa, Ishikawa Takenori
Department of Oral and Maxillofacial Surgery, Graduate School of Biomedical Sciences, Hiroshima University, Kasumi 1-2-3, Minami-Ku, Hiroshima 734-8553, Japan.
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2003 May;95(5):594-600. doi: 10.1067/moe.2003.36.
Our aim was to clarify the association of human papillomavirus (HPV) with oral carcinogenesis, especially its early stage.
Tissue specimens of normal mucosa, epithelial dysplasia, oral squamous cell carcinoma (OSCC), and OSCC cell lines were examined for the presence of HPV-16 and HPV-18 E6 DNA by means of the polymerase chain reaction test.
The detection rate of HPV-16 in epithelial dysplasia (31/51) was higher than that in normal mucosa (16/44) and in OSCC (30/86) and was statistically different from that in OSCC. The cases that progressed from epithelial dysplasia to carcinoma showed a significantly higher HPV-16 detection rate than the other cases in both epithelial dysplasia and OSCC. HPV-16 and HPV-18 were detected only at early passages of 2 of 10 OSCC cell lines.
These results strongly suggest that HPV-16 may be involved in the early stages of the development of some oral carcinomas.
我们的目的是阐明人乳头瘤病毒（HPV）与口腔癌发生的关联，尤其是其早期阶段。
通过聚合酶链反应检测，对正常黏膜、上皮发育异常、口腔鳞状细胞癌（OSCC）及OSCC细胞系的组织标本进行HPV - 16和HPV - 18 E6 DNA检测。
上皮发育异常中HPV - 16的检出率（31/51）高于正常黏膜（16/44）和OSCC（30/86），且与OSCC中的检出率有统计学差异。从上皮发育异常进展为癌的病例，在上皮发育异常和OSCC中，HPV - 16检出率均显著高于其他病例。在10个OSCC细胞系中，仅2个细胞系的早期传代中检测到HPV - 16和HPV - 18。
这些结果有力地表明，HPV - 16可能参与了某些口腔癌发生的早期阶段。
