Kansky A A, Poljak M, Seme K, Kocjan B J, Gale N, Luzar B, Golouh R
Department of Maxillofacial and Oral Surgery, University Medical Center, Ljubljana, Slovenia.
Acta Virol. 2003;47(1):11-6.
To elucidate the putative etiologic role of human papillomaviruses (HPV) in oral carcinogenesis, a comparative study was carried out on 62 tissue specimens of oral squamous cell carcinoma (OSCC) and on 62 specimens of histologically normal oral mucosa obtained from the individuals who matched the subjects with OSCC in age, gender, localization of obtained tissue specimens, drinking and smoking habits. Internal control amplification showed that amplifiable DNA was recovered from 59/62 and 61/62 tissue samples of OSCC and normal oral mucosa, respectively. The amplification with two different HPV L1 and one HPV E6 consensus primer sets showed the presence of the HPV DNA genotypes 16, 33, 58 in 5/59 (8.4%) OSCC specimens and HPV genotypes 11, 16, 31, 68 in 4/61 (6.6%) tissue samples of normal oral mucosa tested. In the study in which a comparative examination of the presence of HPV DNA was for the first time performed on the tissue samples of the patients with OSCC and the age- and gender-matched control subjects there was no significant difference in the prevalence of HPV DNA among both study groups. Our results suggest that occasional findings of HPV DNA in OSCC tissue specimens may be the result of an incidental HPV colonization of oral mucosa, rather than of viral infection, and that HPVs play a limited role in the etiopathogenesis of the majority of OSCC.
为阐明人乳头瘤病毒（HPV）在口腔癌发生中的假定病因作用，对62例口腔鳞状细胞癌（OSCC）组织标本以及从年龄、性别、获取组织标本的部位、饮酒和吸烟习惯与OSCC患者相匹配的个体中获取的62例组织学正常的口腔黏膜标本进行了一项比较研究。内部对照扩增显示，分别从59/62例OSCC组织样本和61/62例正常口腔黏膜组织样本中回收了可扩增DNA。用两种不同的HPV L1和一种HPV E6共有引物组进行扩增，结果显示在检测的5/59例（8.4%）OSCC标本中存在HPV DNA基因型16、33、58，在61例正常口腔黏膜组织样本的4/61例（6.6%）中存在HPV基因型11、16、31、68。在首次对OSCC患者的组织样本与年龄和性别匹配的对照受试者进行HPV DNA存在情况比较检查的研究中，两个研究组中HPV DNA的患病率没有显著差异。我们的结果表明，在OSCC组织标本中偶尔发现HPV DNA可能是口腔黏膜偶然HPV定植的结果，而非病毒感染的结果，并且HPV在大多数OSCC的病因发病机制中起有限作用。
