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116株不相关金黄色葡萄球菌分离株中grlA、grlB、gyrA和gyrB基因突变特征及突变对环丙沙星最低抑菌浓度的影响

Characterization of grlA, grlB, gyrA, and gyrB mutations in 116 unrelated isolates of Staphylococcus aureus and effects of mutations on ciprofloxacin MIC.

作者信息

Schmitz F J, Jones M E, Hofmann B, Hansen B, Scheuring S, Lückefahr M, Fluit A, Verhoef J, Hadding U, Heinz H P, Köhrer K

机构信息

Institute for Medical Microbiology and Virology, Heinrich-Heine University Düsseldorf, Germany.

出版信息

Antimicrob Agents Chemother. 1998 May;42(5):1249-52. doi: 10.1128/AAC.42.5.1249.

DOI:10.1128/AAC.42.5.1249
PMID:9593159
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC105791/
Abstract

One hundred sixteen unrelated clinical isolates of Staphylococcus aureus (70 ciprofloxacin resistant and 46 ciprofloxacin susceptible) from eight countries were studied for the presence of mutations in the grlA, grlB, gyrA, and gyrB gene loci. Two mutations within grlA (located at codons 80 and 84) and two mutations within gyrA (located at codons 84 and 88) were clearly associated with ciprofloxacin resistance, although other mutations detected within the four genes studied may also contribute to decreased susceptibility.

摘要

对来自八个国家的116株无亲缘关系的金黄色葡萄球菌临床分离株（70株对环丙沙星耐药，46株对环丙沙星敏感）进行研究，以检测grlA、grlB、gyrA和gyrB基因位点的突变情况。grlA内的两个突变（位于第80和84密码子）以及gyrA内的两个突变（位于第84和88密码子）与环丙沙星耐药明显相关，尽管在所研究的四个基因中检测到的其他突变也可能导致敏感性降低。

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Specific information concerning taxonomy, pathogenicity and methicillin resistance of staphylococci obtained by a multiplex PCR.通过多重聚合酶链反应获得的有关葡萄球菌分类学、致病性和耐甲氧西林情况的具体信息。
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