Liao S K, Perng Y P, Shen Y C, Chung P J, Chang Y S, Wang C H
Graduate Institute of Clinical Medicine, College of Medicine, Chang Gung University, Taoyuan, Taiwan, Republic of China.
Cancer Genet Cytogenet. 1998 May;103(1):52-8. doi: 10.1016/s0165-4608(97)00416-0.
An epithelial cell line, NPC-BM1, was established from a bone marrow biopsy of a female Taiwanese patient with nasopharyngeal carcinoma (NPC). Histopathology of the bone marrow biopsy and xenografts grown in severe combined immunodeficiency mice showed that the tumor was a nonkeratinizing, poorly differentiated carcinoma. NPC-BM1 cells grown as monolayers had a doubling time of 28.5 hours. Chromosome analysis showed that NPC-BM1 had the following features: 1) hypotetraploidy with a modal chromosome number of 87 (84-90); 2) numerically and structurally normal chromosomes 18; 3) numerical abnormalities without apparent structural alterations on chromosomes 14, 16, 17, 19, and 20; 4) ten structural abnormalities, t(1;9)(p11;q11), t(3;?;4)(p13;?;q13), add(4p),del(6p), i(8) [corrected] (q10),der(?)t(?;12),(?;p12),[corrected] add(21)(p11), del(X)(q24), add(X)(q22), and marker 1 (M1), in all metaphases examined, which were found to be present in two to five cell lines from primary NPC tumors reported previously; and 5) four other abnormalities, t(2;?;2)(p11.2;?;q21),t(11;22)(q11;q11),i(22)(q10), and marker 2 (M2), unique to this metastatic cell line. To the best of our knowledge, NPC-BM1 is the first NPC cell line derived from a distant metastatic site. Further evaluation of this cell line and additional metastatic NPC cell lines as well as primary NPC cell lines with respect to relations between the timing, karyotypic anomalies, and immunobiological characteristics in NPC progression and metastasis is warranted.
一株上皮细胞系NPC - BM1是从一名患鼻咽癌（NPC）的台湾女性患者的骨髓活检样本中建立的。骨髓活检样本及在严重联合免疫缺陷小鼠体内生长的异种移植物的组织病理学检查显示，该肿瘤为非角化性低分化癌。以单层形式生长的NPC - BM1细胞的倍增时间为28.5小时。染色体分析表明，NPC - BM1具有以下特征：1）亚四倍体，众数染色体数为87（84 - 90）；2）18号染色体在数量和结构上正常；3）14、16、17、19和20号染色体存在数量异常但无明显结构改变；4）在所有检查的中期相中存在10种结构异常，即t(1;9)(p11;q11)、t(3;?;4)(p13;?;q13)、add(4p)、del(6p)、i(8)(q10)、der(?)t(?;12),(?;p12)、add(21)(p11)、del(X)(q24)、add(X)(q22)和标记物1（M1），这些异常在先前报道的原发性NPC肿瘤的两到五个细胞系中也有发现；5）另外4种异常，即t(2;?;2)(p11.2;?;q21)、t(11;22)(q11;q11)、i(22)(q10)和标记物2（M2），是该转移细胞系所特有的。据我们所知，NPC - BM1是首个源自远处转移部位的NPC细胞系。有必要进一步评估该细胞系以及其他转移性NPC细胞系和原发性NPC细胞系在NPC进展和转移过程中的时间、核型异常及免疫生物学特征之间的关系。
