基于它们的RecA序列，嗜热绿菌（Chlorobium tepidum）和橙黄嗜热栖热菌（Chloroflexus aurantiacus）的系统发育关系。
The phylogenetic relationships of Chlorobium tepidum and Chloroflexus aurantiacus based upon their RecA sequences.
作者信息
Gruber T M, Eisen J A, Gish K, Bryant D A
机构信息
Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park 16802, USA.
出版信息
FEMS Microbiol Lett. 1998 May 1;162(1):53-60. doi: 10.1111/j.1574-6968.1998.tb12978.x.
Abstract
Using RecA as the phylogenetic marker, the relationships of the green sulfur bacterium Chlorobium tepidum and the green non-sulfur bacterium Chloroflexus aurantiacus to other eubacteria were investigated. The recA genes of the two organisms were cloned, and the resulting protein sequences aligned with 86 other eubacterial RecA sequences. Cb. tepidum was placed as the nearest relative to the Cytophaga/Flexibacter/Bacteriodes group, a relationship supported by results obtained with several phylogenetic markers. Cf. aurantiacus was placed near Chlamydia trachomatis and the high-GC Gram-positives; however, this branching pattern was not strongly supported statistically by bootstrap analyses. Possible reasons for this ambiguity are discussed.
摘要
以RecA作为系统发育标记，研究了嗜热绿硫菌（Chlorobium tepidum）和嗜热栖热放线菌（Chloroflexus aurantiacus）与其他真细菌之间的关系。克隆了这两种生物的recA基因，并将所得蛋白质序列与其他86个真细菌RecA序列进行比对。嗜热栖热放线菌被确定为噬细胞菌属/屈挠杆菌属/拟杆菌属（Cytophaga/Flexibacter/Bacteriodes）组的最近亲缘种，这一关系得到了几种系统发育标记结果的支持。嗜热栖热放线菌被定位在沙眼衣原体（Chlamydia trachomatis）和高GC含量革兰氏阳性菌附近；然而，这种分支模式在统计学上并未得到自展分析的有力支持。文中讨论了造成这种不明确性的可能原因。
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