O'Brien A J, Young H M, Povey J M, Furness J B
Department of Anatomy and Cell Biology, University of Melbourne, Parkville, Victoria, Australia.
Histochem Cell Biol. 1995 Mar;103(3):221-5. doi: 10.1007/BF01454027.
The localization of nitric oxide synthase (NOS) in vascular endothelial cells of submucosal blood vessels from the guinea-pig ileum was examined using NADPH diaphorase histochemistry at the light microscopic level, and endothelial NOS immunohistochemistry at the light and electron microscopic level. The pattern of staining observed following NADPH diaphorase histochemistry and endothelial NOS immunohistochemistry was identical. Endothelial cells of the arterioles, capillaries and venules showed small patches of intense, perinuclear staining. Under the electron microscope, endothelial NOS immunoreactivity was found predominantly in association with the Golgi apparatus and with the membranes of some vesicles. Small regions of the plasma membrane and the rough endoplasmic reticulum also showed some immunoreactivity. The presence of NOS in the Golgi apparatus and in vesicles raises the possibility that NOS may be exteriorized by endothelial cells, and hence that nitric oxide is synthesized extracellularly.
利用光镜水平的NADPH黄递酶组织化学方法以及光镜和电镜水平的内皮型一氧化氮合酶免疫组织化学方法，研究了豚鼠回肠黏膜下血管内皮细胞中一氧化氮合酶（NOS）的定位。NADPH黄递酶组织化学和内皮型一氧化氮合酶免疫组织化学观察到的染色模式相同。小动脉、毛细血管和小静脉的内皮细胞显示出核周小片状的强烈染色。在电子显微镜下，内皮型一氧化氮合酶免疫反应主要与高尔基体和一些囊泡膜相关。质膜和粗面内质网的小区域也显示出一些免疫反应。高尔基体和囊泡中存在NOS增加了NOS可能被内皮细胞外排的可能性，因此一氧化氮是在细胞外合成的。
