Brevet A, Pinto E, Peacock J, Stockdale F E
Science. 1976 Sep 17;193(4258):1152-4. doi: 10.1126/science.959833.
When cultures of skeletal muscle cells of the chick embryo are subjected to repetitive, electrical stimulation, the contractions increase the amount of protein produced by these cells. The increase is greater for contractile proteins such as myosin heavy chain than for total cellular protein. This demonstrates that in a culture system of skeletal muscle cells that have differentiated in the absence of innervation, one can elicit the protein synthetic response associated with skeletal muscle hypertrophy in vivo.
当对鸡胚骨骼肌细胞培养物进行重复性电刺激时，收缩会增加这些细胞产生的蛋白量。对于收缩蛋白如肌球蛋白重链来说，这种增加比对总细胞蛋白的增加更为显著。这表明，在未受神经支配而分化的骨骼肌细胞培养系统中，可以引发与体内骨骼肌肥大相关的蛋白质合成反应。
