Kagawa Y, Ohosaki A, Ohosaki R, Katsuta O, Tsuchitani M, Taniyama H
Vet Pathol. 1998 May;35(3):220-2. doi: 10.1177/030098589803500308.
A testicular yolk sac carcinoma (YSCA) was diagnosed in a 28-day-old male Japanese black calf. Macroscopically, the abdominal cavity was filled with reddish gelatinous masses of various sizes. There were no testes in the scrotum or pelvic cavity. Histologically, the masses consisted of the tissues showing a variety of patterns; loose reticular network, pseudopapillary arrangement, festoon, solid nest, and labyrinthine pattern. Tumor cells were round to oval, with single central or polar nuclei with sharply defined nuclear borders and deeply basophilic chromatin. Tumor cells sometimes had glycogen granules, periodic acid-Schiff (PAS)-positive inclusions, or sudanophilic droplets in their cytoplasm. Hyaline matrices were found in extracellular areas and were intensely PAS positive. Immunohistochemically, most of the tumor cells and hyaline matrices had positive reactivity to anti-alpha-fetoprotein and anti-placental-alkaline-phosphatase antibodies. Positive reaction to anti-laminin antibody appeared only in hyaline matrices. This is the first case of a tumor in a domestic animal with histologic and immunohistochemical features analogous to those of human YSCA, endodermal sinus type.
一头28日龄的雄性日本黑牛犊被诊断患有睾丸卵黄囊癌（YSCA）。大体上，腹腔内充满了各种大小的红色胶冻状肿块。阴囊或盆腔内未发现睾丸。组织学上，肿块由呈现多种模式的组织构成；疏松的网状结构、假乳头排列、花彩状、实性巢状和迷路状模式。肿瘤细胞呈圆形至椭圆形，具有单个中央或极位核，核边界清晰，染色质深嗜碱性。肿瘤细胞的细胞质中有时含有糖原颗粒、过碘酸希夫（PAS）阳性内含物或嗜苏丹性小滴。在细胞外区域发现了透明基质，且PAS染色呈强阳性。免疫组织化学分析显示，大多数肿瘤细胞和透明基质对抗甲胎蛋白和抗胎盘碱性磷酸酶抗体呈阳性反应。抗层粘连蛋白抗体的阳性反应仅出现在透明基质中。这是首例在家畜中发现的具有与人类内胚窦型YSCA相似组织学和免疫组织化学特征的肿瘤病例。
