克隆的17个核苷酸长的合成乳糖操纵基因具有生物活性。

Cloned seventeen-nucleotide-long synthetic lactose operator is biologically active.

作者信息

Bahl C P, Wu R, Narang S

出版信息

Gene. 1978 Apr;3(2):123-34. doi: 10.1016/0378-1119(78)90056-2.

DOI:10.1016/0378-1119(78)90056-2

PMID:95988

Abstract

A 17-nucleotide-long synthetic DNA molecule constituting the minimal recognition sequence of the lactose operator has been cloned in E. coli using the vehicle pBR313 and a synthetic HindIII adaptor. The clones containing the lac-pBR313 hybrid DNA constitutively produced beta-galactosidase. The level of beta-galactosidase was high and comparable to that obtained in cells carrying a 21-nucleotide-long synthetic lac operator on pMB9 plasmid or cells carrying a natural lac operator on pOP203-1 plasmid.

摘要

一个由17个核苷酸组成的构成乳糖操纵子最小识别序列的合成DNA分子，已使用载体pBR313和合成的HindIII接头克隆到大肠杆菌中。含有lac-pBR313杂交DNA的克隆组成型产生β-半乳糖苷酶。β-半乳糖苷酶的水平很高，与在携带pMB9质粒上21个核苷酸长的合成lac操纵子的细胞或携带pOP203-1质粒上天然lac操纵子的细胞中获得的水平相当。

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