Sadler J R, Tecklenburg M, Betz J L, Goeddel D V, Yansura D G, Caruthers M H
Gene. 1977 Jul;1(5-6):305-21. doi: 10.1016/0378-1119(77)90036-1.
Recombinant DNA molecules, constructed from the ColE1-Mk5 hybrid plasmid PMB9 and a chemically synthesized wild-type lactose operator segment, have been used to transform Escherichia coli. Up to 10% of the transformants (selected for the tetracycline-resistance property of PMB9) are partially constitutive for the lactose operon enzyme beta-galactosidase. In vitro studies demonstrate that these partially constitutive transformants contain plasmid DNA molecules which carry one or more lactose operators, and which will bind purified lactose repressor. Preliminary results with some modified operator sequences are also presented.
由ColE1-Mk5杂种质粒PMB9和化学合成的野生型乳糖操纵基因片段构建的重组DNA分子已被用于转化大肠杆菌。高达10%的转化子（根据PMB9的四环素抗性特性筛选）对乳糖操纵子酶β-半乳糖苷酶呈部分组成型。体外研究表明，这些部分组成型转化子含有携带一个或多个乳糖操纵基因且能结合纯化乳糖阻遏物的质粒DNA分子。还给出了一些修饰操纵基因序列的初步结果。
