克隆的合成乳糖操纵基因DNA具有生物活性。
Cloned synthetic lac operator DNA is biologically active.
作者信息
Marians K J, Wu R, Stawinski J, Hozumi T, Narang S A
出版信息
Nature. 1976 Oct 28;263(5580):744-8. doi: 10.1038/263744a0.
DOI:10.1038/263744a0
PMID:792711
Abstract
A chemically synthesised duplex DNA fragment containing the sequence of the lac operator was cloned in E. coli using the vehicle pMB9. Clones containing lac-pMB9 hybrid DNA produced beta-galactosidase constitutively and the hybrid DNA bound the lac repressor specifically.
摘要
一个含有乳糖操纵子序列的化学合成双链DNA片段，利用载体pMB9在大肠杆菌中进行克隆。含有乳糖-pMB9杂交DNA的克隆持续产生β-半乳糖苷酶，且该杂交DNA能特异性结合乳糖阻遏物。
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