Takamatsu J, Tsuda F, Okudaira M
Medical Examiner's Office, Tokyo Metropolitan Government, Japan.
J Med Virol. 1998 Jun;55(2):123-8.
Markers of GB virus C (GBV-C), hepatitis C virus (HCV) and hepatitis B virus (HBV) were sought in sera from 1,044 cases autopsied at the Medical Examiner's Office, Tokyo Metropolitan Government. GBV-C RNA was detected in 35 (3%) cases at a frequency significantly higher (P < 0.05) than in blood donors in Tokyo (4 of 448 or 1%). Three genotypes of GBV-C provisionally designated G1, G2 and G3 were determined by selective amplification with type-specific primers, and G3 (Asian type) was detected in 31 (89%), G2 (European/American type represented by the prototype hepatitis G virus) in three (9%) and G1 (West African type represented by the prototype GBV-C) in one (3%). Antibody to HCV (anti-HCV) was detected in 116 (11%) cases and accompanied by HCV RNA in 88. HCV genotypes were I/1a in one (1%), II/1b in 55 (63%), III/2a in 17 (19%) and IV/2b in 13 (15%). Antibodies to hepatitis B virus (HBV) was detected in 335 (32%) cases and hepatitis B surface antigen in 14 (1%). Subtypes were determined in 12 of them, adw was found in seven (58%), adr in four (33%) and adyr in one (8%). GBV-C RNA was detected significantly more frequently (P< 0.01) in the cases with liver disease (9 of 70 or 13%) than in those with the other causes of death (26 of 974 or 3%). Anti-HCV was more frequent in the cases with GBV-C RNA than in those without it (15 of 35 or 43% vs. 101 of 1,009 or 10%, P< 0.001). These results indicate that infection with GBV-C as well as HCV was common, while infection with HBV was not common in the Medical Examiner's autopsy cases in Tokyo.
在东京都政府法医办公室对1044例尸检病例的血清中检测了GB病毒C（GBV-C）、丙型肝炎病毒（HCV）和乙型肝炎病毒（HBV）的标志物。在35例（3%）病例中检测到GBV-C RNA，其频率显著高于东京的献血者（448例中的4例，即1%，P<0.05）。通过用型特异性引物进行选择性扩增确定了GBV-C的三种基因型，暂定为G1、G2和G3，其中G3（亚洲型）在31例（89%）中检测到，G2（以丙型肝炎G病毒原型为代表的欧美型）在3例（9%）中检测到，G1（以GBV-C原型为代表的西非型）在1例（3%）中检测到。116例（11%）病例中检测到抗HCV（anti-HCV），其中88例伴有HCV RNA。HCV基因型为I/1a的1例（1%）、II/1b的55例（63%）、III/2a的17例（19%）和IV/2b的13例（15%）。335例（32%）病例中检测到乙型肝炎病毒（HBV）抗体，14例（1%）检测到乙型肝炎表面抗原。其中12例确定了亚型，发现adw有7例（58%）、adr有4例（33%）、adyr有1例（8%）。与其他死因的病例（974例中的26例，即3%）相比，GBV-C RNA在肝病病例中检测到的频率显著更高（70例中的9例，即13%，P<0.01）。与未感染GBV-C RNA的病例相比，感染GBV-C RNA的病例中anti-HCV更常见（35例中的15例，即43%，而1009例中的101例，即10%，P<0.001）。这些结果表明，在东京法医尸检病例中，GBV-C以及HCV感染很常见，而HBV感染并不常见。
