Yu M L, Chuang W L, Dai C Y, Lu S N, Wang J H, Huang J F, Chen S C, Lin Z Y, Hsieh M Y, Tsai J F, Wang L Y, Chang W Y
Hepatobiliary Division, Department of Internal Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan.
J Infect. 2001 Jan;42(1):61-6. doi: 10.1053/jinf.2000.0785.
To investigate the serological and molecular characteristics of GB virus C/hepatitis G virus (GBV-C/HGV) infection in the hepatitis C virus (HCV)/hepatitis B virus (HBV)-endemic areas in Taiwan.
Sera from 200 residents from Masago, an HCV/HBV-endemic community in Taiwan, and 400 blood donors were tested for GBV-C/HGV RNA by using nested reverse transcription-polymerase chain reaction and for antibodies to GBV-C/HGV E2-protein (anti-E2) by an enzyme-linked immunosorbent assay. Phylogenetic analysis of GBV-C/HGV was performed.
The prevalence of GBV-C/HGV viraemia, anti-E2 and GBV-C/HGV exposure among residents of Masago was significantly higher than that among donors (17.0%, 25.5% and 39.5% vs. 3.3%, 7.5% and 10.3%, respectively; all P < 0.0001). In Masago, the prevalence of GBV-C/HGV exposure was significantly higher in residents exposed to HCV than in those without HCV exposure (45.8% vs. 24.1%;P< 0.005). Based on multivariate analyses, HCV viraemia was the only significant factor associated with elevated levels of alanine aminotransferase in Masago. Phylogenetic analysis showed all 34 GBV-C/HGV isolates from Masago clustered within genotype 3.
GBV-C/HGV was highly prevalent in Masago, an HCV/HBV-endemic community in Taiwan. HCV viraemia played the most important clinical hepatopathic role in the area. Infections with other hepatitis viruses did not influence the anti-E2 seroconversion from GBV-C/HGV infections.
调查台湾丙型肝炎病毒（HCV）/乙型肝炎病毒（HBV）流行地区庚型肝炎病毒（GBV-C/HGV）感染的血清学和分子特征。
采用巢式逆转录-聚合酶链反应检测台湾HCV/HBV流行社区马沙沟200名居民和400名献血者血清中的GBV-C/HGV RNA，采用酶联免疫吸附试验检测GBV-C/HGV E2蛋白抗体（抗-E2）。对GBV-C/HGV进行系统发育分析。
马沙沟居民中GBV-C/HGV病毒血症、抗-E2和GBV-C/HGV暴露的患病率显著高于献血者（分别为17.0%、25.5%和39.5%，而献血者分别为3.3%、7.5%和10.3%；所有P<0.0001）。在马沙沟，暴露于HCV的居民中GBV-C/HGV暴露的患病率显著高于未暴露于HCV的居民（45.8%对24.1%；P<0.005）。基于多变量分析，HCV病毒血症是马沙沟丙氨酸氨基转移酶水平升高的唯一显著相关因素。系统发育分析显示，来自马沙沟的所有34株GBV-C/HGV分离株均聚集在3型基因型内。
GBV-C/HGV在台湾HCV/HBV流行社区马沙沟高度流行。HCV病毒血症在该地区发挥了最重要的临床肝病作用。其他肝炎病毒感染不影响GBV-C/HGV感染的抗-E2血清转化。
