Janebová M, Zima T
Institute of Medical Chemistry and Biochemistry, 1st Faculty of Medicine, Charles University, Prague, Czech Republic.
Sb Lek. 1997;98(3):195-208.
Biological effects of reactive oxygen species and other radicals which are controlled by antioxidant mechanism are exerted on the basis of enzymes and substrates. Antioxidant substrates are divided into lipophilic and hydrophilic groups. Main representants of lipophilic antioxidants are retinol and tocopherol which are closely related to ascorbate in the hydrophilic compartment. Described methods for detection of retinol and alpha-tocopherol are simple, sensitive, specific and precise. They are reliable, economic and fast. Only small amounts of serum are used for one analysis which is convenient for patients, too. Our methods can be recommended for routine use in laboratories of clinical biochemistry. Haemodialyzed patients, retinol serum level was higher than that in blood donors while alpha-tocopherol serum level did not differ. Both vitamin A and E serum levels were similar in alcoholics compared to controls.
由抗氧化机制控制的活性氧和其他自由基的生物学效应是基于酶和底物发挥作用的。抗氧化底物分为亲脂性和亲水性两类。亲脂性抗氧化剂的主要代表是视黄醇和生育酚，它们与亲水性隔室中的抗坏血酸密切相关。所描述的视黄醇和α-生育酚的检测方法简单、灵敏、特异且精确。它们可靠、经济且快速。一次分析仅需使用少量血清，这对患者来说也很方便。我们的方法可推荐用于临床生物化学实验室的常规使用。血液透析患者的血清视黄醇水平高于献血者，而血清α-生育酚水平无差异。与对照组相比，酗酒者的维生素A和E血清水平相似。
