Sørensen S J, Jensen L E
Department of General Microbiology, University of Copenhagen, Denmark.
Antonie Van Leeuwenhoek. 1998 Jan;73(1):69-77. doi: 10.1023/a:1000661115753.
Transfer of plasmid RP4 to indigenous bacteria in bulk soil could only be detected in soil with nutrient amendment. Lack of physiological active donor and recipient cells was apparently one of the limiting factors in un-amended bulk soil. Plasmid transfer was detected both in the spermosphere and rhizosphere of barley seedlings. Transfer occurred from seed coated donor bacteria (i) to introduced recipient bacteria and (ii) to indigenous bacteria present in soil. Plasmid transfer was also detected from donor bacteria introduced to the soil to seed coated recipient bacteria. Transfer efficiencies in the rhizosphere were significantly below the transfer efficiencies obtained in the spermosphere. The transfer efficiencies detected in the barley spermosphere were among the highest reported from any natural environment.
只有在添加营养物质的土壤中才能检测到质粒RP4向大量土壤中土著细菌的转移。缺乏生理活性的供体细胞和受体细胞显然是未添加营养物质的大量土壤中的限制因素之一。在大麦幼苗的根际和根圈中均检测到了质粒转移。转移发生在以下情况：（i）从包衣种子的供体细菌转移到引入的受体细菌；（ii）从包衣种子的供体细菌转移到土壤中存在的土著细菌。还检测到从引入土壤的供体细菌向包衣种子的受体细菌的质粒转移。根际中的转移效率明显低于在根圈中获得的转移效率。在大麦根圈中检测到的转移效率是任何自然环境中报道的最高效率之一。
