Picolo Marta, Stephen Abish, Baysan Aylin
Centre for Oral Bioengineering, Institute of Dentistry, Barts and The London School of Medicine and Dentistry, Queen Mary University of London, London, UK.
Centre for Oral Immunobiology and Regenerative Medicine, Institute of Dentistry, Barts and The London School of Medicine and Dentistry, Queen Mary University of London, London, UK.
J Oral Microbiol. 2024 Mar 27;16(1):2327758. doi: 10.1080/20002297.2024.2327758. eCollection 2024.
is a virulent microorganism associated with dental caries. This study aimed to investigate the antimicrobial effects of Cholecalciferol (D3) and Doxercalciferol (D2), against and on glycosyltransferase gene expression.
Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of D3 and D2 for were determined according to the Clinical Laboratory Standards Institute guidelines. The effect of the compounds on environmental pH in 1% w/v and 5% w/v sucrose broth cultures after 24 hours were assessed colorimetrically. Additionally, their impact on glycosyltransferases gene expression () in 5% w/v sucrose culture was evaluated using quantitative real-time PCR.
The MBCs of D3 and D2 were 83 µg/ml and 166 µg/ml respectively. Both compounds were effective in preventing the local pH drop <5.5 at ≥166 µg/ml in sucrose supplemented cultures. However, the compounds did not inhibit pH drop at MIC values. Notably, D2 upregulated expression significantly ( < 0.05) and downregulated and .
Vitamin D2 and D3 inhibited mediated pH drop in sucrose supplemented cultures and altered glycosyltransferase expression, suggesting potential therapeutic roles in dental caries prevention. Further research is needed to assess their full impact on survival under environmental stresses.
是一种与龋齿相关的致病性微生物。本研究旨在调查胆钙化醇（D3）和度骨化醇（D2）对其抗菌作用以及对糖基转移酶基因表达的影响。
根据临床实验室标准协会指南测定D3和D2对的最低抑菌浓度（MIC）和最低杀菌浓度（MBC）。通过比色法评估化合物在24小时后对1% w/v和5% w/v蔗糖肉汤培养物中环境pH的影响。此外，使用定量实时PCR评估它们对5% w/v蔗糖培养物中糖基转移酶基因表达（）的影响。
D3和D2的MBC分别为83μg/ml和166μg/ml。在添加蔗糖的培养物中，两种化合物在≥166μg/ml时均能有效防止局部pH降至<5.5。然而，在MIC值时，化合物并未抑制pH下降。值得注意的是，D2显著上调表达（<0.05），并下调和。
维生素D2和D3在添加蔗糖的培养物中抑制介导的pH下降，并改变糖基转移酶表达，提示在预防龋齿方面具有潜在治疗作用。需要进一步研究以评估它们在环境压力下对生存的全面影响。
