Singh S, Poulsom R, Hanby A M, Rogers L A, Wright N A, Sheppard M C, Langman M J
Department of Medicine, Queen Elizabeth Hospital, Edgbaston, Birmingham, U.K.
J Pathol. 1998 Feb;184(2):153-60. doi: 10.1002/(SICI)1096-9896(199802)184:2<153::AID-PATH993>3.0.CO;2-W.
Since there is a preponderance of large bowel cancer in males, both in humans and in experimental models, and hormone replacement therapy is protective, a role for sex steroid hormones in the pathogenesis of this neoplasm seems likely. Evidence of functional oestrogen receptor has been looked for in large bowel mucosa and cancer. Expression of oestrogen receptor, and of the oestrogen-inducible receptor-associated genes pS2 and ERD5, was sought. Oestrogen receptor mRNA was detected in cancers and paired normal mucosae in equal amounts. In situ hybridization identified stromal cells above the muscularis mucosae that were positive for oestrogen receptor mRNA. pS2 mRNA was also detected, with a signal intensity significantly higher in normal mucosa compared with cancers, whereas the reverse was seen with ERD5 mRNA levels. pS2 and ERD5 were expressed in epithelium, with the former in a greater amount in distal colon and rectum than proximal colon. Although oestrogen-inducible and receptor-associated genes are expressed in large bowel mucosa, their expression does not correlate with oestrogen receptor.
由于在人类和实验模型中，男性的大肠癌发病率都较高，而激素替代疗法具有保护作用，因此性类固醇激素在这种肿瘤的发病机制中似乎发挥了作用。人们一直在大肠黏膜和癌症中寻找功能性雌激素受体的证据。研究人员探寻了雌激素受体以及雌激素诱导的受体相关基因pS2和ERD5的表达情况。在癌症组织和配对的正常黏膜中检测到等量的雌激素受体mRNA。原位杂交鉴定出黏膜肌层上方的基质细胞雌激素受体mRNA呈阳性。还检测到了pS2 mRNA，其在正常黏膜中的信号强度明显高于癌症组织，而ERD5 mRNA水平则相反。pS2和ERD5在上皮中表达，前者在远端结肠和直肠中的表达量高于近端结肠。虽然雌激素诱导的和受体相关的基因在大肠黏膜中表达，但其表达与雌激素受体并无关联。
