Singh S, Baker P R, Poulsom R, Wright N A, Sheppard M C, Langman M J, Neoptolemos J P
Department of Medicine, University of Birmingham, Queen Elizabeth Hospital, UK.
Br J Surg. 1997 Aug;84(8):1085-9.
Previous studies have not been able to demonstrate convincingly whether the human pancreas expresses oestrogen receptor and whether there is any benefit from antioestrogen therapy in advanced pancreatic cancer.
Oestrogen receptor expression was assessed in normal human pancreas and pancreatic cancer tissue by enzyme immunoassay, Northern blot analysis, in situ hybridization and immunohistochemistry. The expression of the oestrogen-inducible proteins, progesterone receptor, pS2 and ERD5 was also examined.
A mean of 1.0 (range 0-2.4) fmol oestrogen receptor per mg protein was detected in normal pancreas and 0.5 (range 0-1.2) fmol mg-1 in pancreatic cancer. Messenger RNA for oestrogen receptor was detected in both normal and cancerous pancreas. In situ hybridization and immunohistochemistry, however, failed to localize oestrogen receptor expression. Mean (range) expression of progesterone receptor in normal and neoplastic pancreas was 1.9 (0.5-3.5) and 2.5 (0.3-9.3) fmol mg-1 respectively. pS2 and ERD5 were also expressed in normal tissue and pancreatic cancer, and expression was localized to ductular epithelium.
The amount of oestrogen receptor detected in pancreatic tissue was small, and may account for previous difficulties in its detection. The extent to which it is functional in both the normal and malignant pancreas warrants further investigation.
既往研究未能令人信服地证明人类胰腺是否表达雌激素受体，以及抗雌激素治疗对晚期胰腺癌是否有任何益处。
通过酶免疫测定、Northern印迹分析、原位杂交和免疫组织化学评估正常人胰腺和胰腺癌组织中的雌激素受体表达。还检测了雌激素诱导蛋白、孕激素受体、pS2和ERD5的表达。
在正常胰腺中检测到每毫克蛋白质平均1.0（范围0 - 2.4）fmol雌激素受体，在胰腺癌中为0.5（范围0 - 1.2）fmol mg⁻¹。在正常胰腺和癌性胰腺中均检测到雌激素受体的信使核糖核酸。然而，原位杂交和免疫组织化学未能定位雌激素受体的表达。正常胰腺和肿瘤性胰腺中孕激素受体的平均（范围）表达分别为1.9（0.5 - 3.5）和2.5（0.3 - 9.3）fmol mg⁻¹。pS2和ERD5也在正常组织和胰腺癌中表达，且表达定位于导管上皮。
在胰腺组织中检测到的雌激素受体量很少，这可能解释了以往检测中的困难。其在正常胰腺和恶性胰腺中的功能程度值得进一步研究。
