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洋葱伯克霍尔德菌L-膦酰丙氨酸矿化菌株中的磷酸烯醇丙酮酸磷酸变位酶活性

Phosphoenolpyruvate phosphomutase activity in an L-phosphonoalanine-mineralizing strain of burkholderia cepacia.

作者信息

Ternan NG, McGrath JW, Quinn JP

机构信息

School of Biology and Biochemistry, The Queen's University of Belfast, Belfast, Northern Ireland.

出版信息

Appl Environ Microbiol. 1998 Jun;64(6):2291-4. doi: 10.1128/AEM.64.6.2291-2294.1998.

Abstract

A strain of Burkholderia cepacia isolated by enrichment culture utilized L-2-amino-3-phosphonopropionic acid (phosphonoalanine) at concentrations up to 20 mM as a carbon, nitrogen, and phosphorus source in a phosphate-insensitive manner. Cells contained phosphoenolpyruvate phosphomutase activity, presumed to be responsible for cleavage of the C---P bond of phosphonopyruvate, the transamination product of L-phosphonoalanine; this was inducible in the presence of phosphonoalanine.

摘要

通过富集培养分离得到的一株洋葱伯克霍尔德菌,能够以对磷酸盐不敏感的方式,利用浓度高达20 mM的L-2-氨基-3-膦酰丙酸(膦酰丙氨酸)作为碳源、氮源和磷源。细胞含有磷酸烯醇式丙酮酸磷酸变位酶活性,推测该酶负责催化膦酰丙酮酸(L-膦酰丙氨酸的转氨产物)中C---P键的断裂;在膦酰丙氨酸存在的情况下,该酶可被诱导产生。

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