编码莠去津分解代谢的atzABC基因位于假单胞菌属菌株ADP的一个自我传递质粒上。
The atzABC genes encoding atrazine catabolism are located on a self-transmissible plasmid in Pseudomonas sp. strain ADP.
作者信息
de Souza M L, Wackett L P, Sadowsky M J
机构信息
Department of Biochemistry, University of Minnesota, St. Paul, Minnesota 55108, USA.
出版信息
Appl Environ Microbiol. 1998 Jun;64(6):2323-6. doi: 10.1128/AEM.64.6.2323-2326.1998.
Abstract
Pseudomonas sp. strain ADP initiates atrazine catabolism via three enzymatic steps, encoded by atzA, -B, and -C, which yield cyanuric acid, a nitrogen source for many bacteria. In-well lysis, Southern hybridization, and plasmid transfer studies indicated that the atzA, -B, and -C genes are localized on a 96-kb self-transmissible plasmid, pADP-1, in Pseudomonas sp. strain ADP. High-performance liquid chromatography analyses showed that cyanuric acid degradation was not encoded by pADP-1. pADP-1 was transferred to Escherichia coli strains at a frequency of 4.7 x 10(-2). This suggests a potential molecular mechanism for the dispersion of the atzABC genes to other soil bacteria.
摘要
假单胞菌属ADP菌株通过由atzA、-B和-C编码的三个酶促步骤启动阿特拉津的分解代谢，产生氰尿酸，这是许多细菌的氮源。孔内裂解、Southern杂交和质粒转移研究表明，atzA、-B和-C基因位于假单胞菌属ADP菌株中一个96 kb的自我传递质粒pADP-1上。高效液相色谱分析表明，氰尿酸的降解不是由pADP-1编码的。pADP-1以4.7×10(-2)的频率转移到大肠杆菌菌株中。这表明atzABC基因向其他土壤细菌扩散的潜在分子机制。
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