根瘤菌属分离株对莠去津的脱氯作用。
Dechlorination of Atrazine by a Rhizobium sp. Isolate.
出版信息
Appl Environ Microbiol. 1997 Mar;63(3):862-6. doi: 10.1128/aem.63.3.862-866.1997.
Abstract
A Rhizobium sp. strain, named PATR, was isolated from an agricultural soil and found to actively degrade the herbicide atrazine. Incubation of PATR in a basal liquid medium containing 30 mg of atrazine liter(sup-1) resulted in the rapid consumption of the herbicide and the accumulation of hydroxyatrazine as the only metabolite detected after 8 days of culture. Experiments performed with ring-labeled [(sup14)C]atrazine indicated no mineralization. The enzyme responsible for the hydroxylation of atrazine was partially purified and found to consist of four 50-kDa subunits. Its synthesis in PATR was constitutive. This new atrazine hydrolase demonstrated 92% sequence identity through a 24-amino-acid fragment with atrazine chlorohydrolase AtzA produced by Pseudomonas sp. strain ADP.
摘要
一株根瘤菌（Rhizobium sp.）菌株，命名为 PATR，从农业土壤中分离得到，被发现能够有效降解莠去津除草剂。将 PATR 菌株在含有 30 毫克莠去津/升的基础液体培养基中孵育，结果表明在 8 天的培养后，莠去津迅速被消耗，且羟化莠去津是唯一被检测到的代谢产物。用环标记的[(sup14)C]莠去津进行的实验表明没有矿化作用。负责莠去津羟基化的酶被部分纯化，并发现它由四个 50kDa 的亚基组成。在 PATR 中，该酶的合成是组成型的。通过与 Pseudomonas sp. strain ADP 产生的莠去津氯水解酶 AtzA 的 24 个氨基酸片段的比较，发现这种新的莠去津水解酶具有 92%的序列同一性。
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