肠致病性大肠杆菌中bfpA表达所需顺式作用元件的分析
Analysis of cis-acting elements required for bfpA expression in enteropathogenic Escherichia coli.
作者信息
Bustamante V H, Calva E, Puente J L
机构信息
Departamento de Microbiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos 62250, Mexico.
出版信息
J Bacteriol. 1998 Jun;180(11):3013-6. doi: 10.1128/JB.180.11.3013-3016.1998.
Abstract
bfpA expression in enteropathogenic Escherichia coli is regulated by growth medium, temperature, and ammonium concentration and requires the BfpT protein (also called PerA), a member of the AraC family of transcriptional activators. Site-directed and PCR random mutagenesis, as well as deletion analysis of the bfpA upstream regulatory region, supported assignment of the promoter elements and demonstrated that the cis-acting elements that mediate BfpT-dependent regulation of bfpA are located between positions -85 and -46. Interestingly, this region shares 73% identity with a 40-bp-long AT-rich tract located upstream of the bfpT gene, which is essential for bfpT autoregulation.
摘要
肠道致病性大肠杆菌中bfpA的表达受生长培养基、温度和铵浓度的调节，并且需要BfpT蛋白（也称为PerA），它是转录激活因子AraC家族的成员。定点诱变和PCR随机诱变，以及bfpA上游调控区的缺失分析，支持了启动子元件的定位，并证明介导bfpA的BfpT依赖性调控的顺式作用元件位于-85至-46位之间。有趣的是，该区域与位于bfpT基因上游的一段40bp长的富含AT的序列有73%的同一性，这对于bfpT的自我调节至关重要。
相似文献
1
Analysis of cis-acting elements required for bfpA expression in enteropathogenic Escherichia coli.肠致病性大肠杆菌中bfpA表达所需顺式作用元件的分析
J Bacteriol. 1998 Jun;180(11):3013-6. doi: 10.1128/JB.180.11.3013-3016.1998.
2
Autoactivation and environmental regulation of bfpT expression, the gene coding for the transcriptional activator of bfpA in enteropathogenic Escherichia coli.肠致病性大肠杆菌中bfpA转录激活因子编码基因bfpT的自激活及环境调控
Mol Microbiol. 1999 Jul;33(1):153-66. doi: 10.1046/j.1365-2958.1999.01460.x.
3
Cloning and characterization of bfpTVW, genes required for the transcriptional activation of bfpA in enteropathogenic Escherichia coli.肠致病性大肠杆菌中bfpA转录激活所需基因bfpTVW的克隆与特性分析
Mol Microbiol. 1996 Sep;21(5):963-75. doi: 10.1046/j.1365-2958.1996.531415.x.
4
Identification of the DNA binding sites of PerA, the transcriptional activator of the bfp and per operons in enteropathogenic Escherichia coli.鉴定肠致病性大肠杆菌中bfp和per操纵子的转录激活因子PerA的DNA结合位点。
J Bacteriol. 2003 May;185(9):2835-47. doi: 10.1128/JB.185.9.2835-2847.2003.
5
A novel chromosomal locus of enteropathogenic Escherichia coli (EPEC), which encodes a bfpT-regulated chaperone-like protein, TrcA, involved in microcolony formation by EPEC.一种新型的肠致病性大肠杆菌（EPEC）染色体位点，其编码一种受bfpT调控的伴侣样蛋白TrcA，该蛋白参与EPEC微菌落的形成。
Mol Microbiol. 1999 Aug;33(4):741-52. doi: 10.1046/j.1365-2958.1999.01522.x.
6
The bundle-forming pili of enteropathogenic Escherichia coli: transcriptional regulation by environmental signals.肠道致病性大肠杆菌的束状菌毛：环境信号的转录调控
Mol Microbiol. 1996 Apr;20(1):87-100. doi: 10.1111/j.1365-2958.1996.tb02491.x.
7
A plasmid-encoded regulatory region activates chromosomal eaeA expression in enteropathogenic Escherichia coli.一种质粒编码的调控区域可激活肠致病性大肠杆菌中染色体eaeA的表达。
Infect Immun. 1995 May;63(5):1767-76. doi: 10.1128/iai.63.5.1767-1776.1995.
8
A plasmid-encoded type IV fimbrial gene of enteropathogenic Escherichia coli associated with localized adherence.一种与局部黏附相关的致病性大肠杆菌的质粒编码IV型菌毛基因。
Mol Microbiol. 1992 Nov;6(22):3427-37. doi: 10.1111/j.1365-2958.1992.tb02210.x.
9
Transcriptional regulation of the orf19 gene and the tir-cesT-eae operon of enteropathogenic Escherichia coli.肠致病性大肠杆菌orf19基因和tir-cesT-eae操纵子的转录调控
J Bacteriol. 2001 May;183(9):2823-33. doi: 10.1128/JB.183.9.2823-2833.2001.
10
Transcriptional regulation of type III secretion genes in enteropathogenic Escherichia coli: Ler antagonizes H-NS-dependent repression.肠致病性大肠杆菌中III型分泌基因的转录调控：Ler拮抗H-NS依赖性抑制作用。
Mol Microbiol. 2001 Feb;39(3):664-78. doi: 10.1046/j.1365-2958.2001.02209.x.
引用本文的文献
1
Highly-conserved regulatory activity of the ANR family in the virulence of diarrheagenic bacteria through interaction with master and global regulators.通过与主调控因子和全局调控因子相互作用，ANR 家族在致泻性细菌毒力中具有高度保守的调控活性。
Sci Rep. 2023 Apr 29;13(1):7024. doi: 10.1038/s41598-023-33997-0.
2
The transcriptional activator of the bfp operon in EPEC (PerA) interacts with the RNA polymerase alpha subunit.EPEC 中 bfp 操纵子的转录激活蛋白（PerA）与 RNA 聚合酶α亚基相互作用。
Sci Rep. 2021 Apr 20;11(1):8541. doi: 10.1038/s41598-021-87586-0.
3
Self-Conjugation of the Enteropathogenic Adherence Factor Plasmid of Four Typical EPEC Isolates.四株典型 EPEC 致病粘附因子质粒的自身连接。
Biomed Res Int. 2017;2017:6325736. doi: 10.1155/2017/6325736. Epub 2017 Oct 26.
4
RpoS role in virulence and fitness in enteropathogenic Escherichia coli.RpoS在肠致病性大肠杆菌毒力和适应性中的作用。
PLoS One. 2017 Jun 29;12(6):e0180381. doi: 10.1371/journal.pone.0180381. eCollection 2017.
5
PerC Manipulates Metabolism and Surface Antigens in Enteropathogenic .经皮穿刺胃造口术改变肠致病性. 的代谢和表面抗原
Front Cell Infect Microbiol. 2017 Feb 7;7:32. doi: 10.3389/fcimb.2017.00032. eCollection 2017.
6
relA enhances the adherence of enteropathogenic Escherichia coli.relA 增强肠致病性大肠杆菌的黏附性。
PLoS One. 2014 Mar 18;9(3):e91703. doi: 10.1371/journal.pone.0091703. eCollection 2014.
7
The E. coli effector protein NleF is a caspase inhibitor.大肠杆菌效应蛋白 NleF 是一种半胱天冬酶抑制剂。
PLoS One. 2013;8(3):e58937. doi: 10.1371/journal.pone.0058937. Epub 2013 Mar 14.
8
Further characterization of functional domains of PerA, role of amino and carboxy terminal domains in DNA binding.进一步鉴定 PerA 的功能域，鉴定氨基端和羧基端结构域在 DNA 结合中的作用。
PLoS One. 2013;8(2):e56977. doi: 10.1371/journal.pone.0056977. Epub 2013 Feb 25.
9
Enteropathogenic and enterohemorrhagic Escherichia coli virulence gene regulation.肠致病性和肠出血性大肠杆菌毒力基因调控
Infect Immun. 2007 Sep;75(9):4199-210. doi: 10.1128/IAI.01927-06. Epub 2007 Jun 18.
10
Identification of the DNA binding sites of PerA, the transcriptional activator of the bfp and per operons in enteropathogenic Escherichia coli.鉴定肠致病性大肠杆菌中bfp和per操纵子的转录激活因子PerA的DNA结合位点。
J Bacteriol. 2003 May;185(9):2835-47. doi: 10.1128/JB.185.9.2835-2847.2003.
本文引用的文献
1
Autoactivation and environmental regulation of bfpT expression, the gene coding for the transcriptional activator of bfpA in enteropathogenic Escherichia coli.肠致病性大肠杆菌中bfpA转录激活因子编码基因bfpT的自激活及环境调控
Mol Microbiol. 1999 Jul;33(1):153-66. doi: 10.1046/j.1365-2958.1999.01460.x.
2
Transcriptional control of genes encoding CS1 pili: negative regulation by a silencer and positive regulation by Rns.编码CS1菌毛的基因的转录调控：由一个沉默子进行负调控以及由Rns进行正调控。
J Bacteriol. 1997 Sep;179(18):5736-43. doi: 10.1128/jb.179.18.5736-5743.1997.
3
Interactions between enteropathogenic Escherichia coli and host epithelial cells.肠道致病性大肠杆菌与宿主上皮细胞之间的相互作用。
Trends Microbiol. 1997 Mar;5(3):109-14. doi: 10.1016/S0966-842X(97)01000-7.
4
BfpB, an outer membrane lipoprotein required for the biogenesis of bundle-forming pili in enteropathogenic Escherichia coli.BfpB是肠道致病性大肠杆菌中形成束状菌毛生物合成所必需的一种外膜脂蛋白。
J Bacteriol. 1996 Nov;178(22):6555-63. doi: 10.1128/jb.178.22.6555-6563.1996.
5
Cloning and characterization of bfpTVW, genes required for the transcriptional activation of bfpA in enteropathogenic Escherichia coli.肠致病性大肠杆菌中bfpA转录激活所需基因bfpTVW的克隆与特性分析
Mol Microbiol. 1996 Sep;21(5):963-75. doi: 10.1046/j.1365-2958.1996.531415.x.
6
The bundle-forming pili of enteropathogenic Escherichia coli: transcriptional regulation by environmental signals.肠道致病性大肠杆菌的束状菌毛：环境信号的转录调控
Mol Microbiol. 1996 Apr;20(1):87-100. doi: 10.1111/j.1365-2958.1996.tb02491.x.
7
A cluster of fourteen genes from enteropathogenic Escherichia coli is sufficient for the biogenesis of a type IV pilus.来自肠致病性大肠杆菌的一组14个基因足以实现IV型菌毛的生物合成。
Mol Microbiol. 1996 Apr;20(2):325-37. doi: 10.1111/j.1365-2958.1996.tb02620.x.
8
Enteropathogenic Escherichia coli: identification of a gene cluster coding for bundle-forming pilus morphogenesis.肠致病性大肠杆菌：编码束状菌毛形态发生的基因簇的鉴定
J Bacteriol. 1996 May;178(9):2613-28. doi: 10.1128/jb.178.9.2613-2628.1996.
9
A third recognition element in bacterial promoters: DNA binding by the alpha subunit of RNA polymerase.细菌启动子中的第三种识别元件：RNA聚合酶α亚基与DNA的结合。
Science. 1993 Nov 26;262(5138):1407-13. doi: 10.1126/science.8248780.
10
Cloning and characterization of the bundle-forming pilin gene of enteropathogenic Escherichia coli and its distribution in Salmonella serotypes.肠致病性大肠杆菌成束菌毛菌素基因的克隆、特性分析及其在沙门氏菌血清型中的分布
Mol Microbiol. 1993 Feb;7(4):563-75. doi: 10.1111/j.1365-2958.1993.tb01147.x.
Zotero 插件