Dolan J W, Snyder L R, Djordjevic N M, Hill D W, Saunders D L, Van Heukelem L, Waeghe T J
LC Resources Inc., Walnut Creek, CA 94596, USA.
J Chromatogr A. 1998 Apr 17;803(1-2):1-31. doi: 10.1016/s0021-9673(97)01293-4.
The optimized reversed-phase HPLC separation of 14 different samples is reported, based on simultaneous changes in temperature and gradient steepness. Four experimental runs are required for each sample, following which preferred conditions can be predicted using computer simulation software (DryLab). The overall accuracy and effectiveness of this method development approach is discussed, with particular attention to the use of resolution maps provided by the software. These maps are useful for maximizing resolution for the total sample, for optimizing the separation of a smaller number of selected sample compounds, and as an initial step in the separation of more demanding samples.
本文报道了基于温度和梯度陡度同时变化对14种不同样品进行优化的反相高效液相色谱分离方法。每个样品需要进行四次实验运行，之后可使用计算机模拟软件（DryLab）预测优选条件。讨论了这种方法开发途径的整体准确性和有效性，特别关注软件提供的分辨率图谱的使用。这些图谱对于最大化整个样品的分辨率、优化较少数量选定样品化合物的分离以及作为分离要求更高样品的初始步骤很有用。
