Dolan J W, Snyder L R, Saunders D L, Van Heukelem L
LC Resources Inc., Walnut Creek, CA 94596, USA.
J Chromatogr A. 1998 Apr 17;803(1-2):33-50. doi: 10.1016/s0021-9673(97)01294-6.
The preceding paper (Part I) suggests that simply optimizing temperature and gradient steepness will often provide an adequate reversed-phase HPLC separation. In some cases, however, this procedure will prove unsuccessful, and then further method-development experiments (involving change in other separation conditions) will be required. One strategy is to change a variable other than temperature or gradient steepness, followed by re-optimization of the latter two variables. The present paper examines the application of this approach with the aid of computer simulation to several samples.
