哺乳动物克隆的最新进展。
Recent progress in mammalian cloning.
作者信息
Wolf D P, Meng L, Ely J J, Stouffer R L
机构信息
Division of Reproductive Sciences, Oregon Regional Primate Research Center, Beaverton 97006-3499, USA.
出版信息
J Assist Reprod Genet. 1998 May;15(5):235-9. doi: 10.1023/a:1022575923493.
PURPOSE
Our purpose was to review recent progress in the use of nuclear transfer technology to produce genetically identical mammals.
METHODS
A literature review was conducted.
RESULTS
The reasons for cloning nonhuman mammals are manyfold including commercial, biomedical, and basic research applications. Individual steps in the nuclear transfer process are itemized, along with a detailed description of the specific approaches used in the production of Dolly, NETI, and DITTO. The potential application of nuclear transfer in the treatment of human infertility is also considered, along with bioethical concerns. Finally, insights are provided concerning the future application of cloning technology in rhesus macaques.
CONCLUSIONS
The cloning of a lamb (Dolly) from an adult, mammary gland cell coupled with the successful production of rhesus monkeys (NETI and DITTO) by nuclear transfer of embryonic cells marks the beginning of a "Golden Age" in the development and application of somatic cell cloning technology in mammals.
目的
我们的目的是回顾利用核移植技术生产基因相同的哺乳动物的近期进展。
方法
进行了文献综述。
结果
克隆非人类哺乳动物的原因是多方面的，包括商业、生物医学和基础研究应用。详细列出了核移植过程中的各个步骤，并对多利、NETI和DITTO的生产中使用的具体方法进行了详细描述。还考虑了核移植在治疗人类不孕症方面的潜在应用以及生物伦理问题。最后，提供了关于克隆技术在恒河猴未来应用的见解。
结论
从成年乳腺细胞克隆出一只小羊（多利），以及通过胚胎细胞核移植成功培育出恒河猴（NETI和DITTO），标志着哺乳动物体细胞克隆技术发展和应用“黄金时代”的开始。
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