Campbell K H S, Alberio R, Choi I, Fisher P, Kelly R D W, Lee J-H, Maalouf W
Animal Development and Biotechnology Group, Division of Animal Physiology, University of Nottingham, School of Biosciences, Sutton Bonington, Loughborough, Leics LE12 5RD, UK.
Reprod Domest Anim. 2005 Aug;40(4):256-68. doi: 10.1111/j.1439-0531.2005.00591.x.
It is now 8 years since the birth of Dolly, the first animal produced by nuclear transfer using a donor cell population established from an adult animal. During this time, the technique of nuclear transfer has been successfully applied to a range of mammalian species for the production of offspring using a plethora of donor cell types derived from both foetal and adult tissues. In addition, when coupled with genetic manipulation of the donor cells, transgenic offspring have been produced with a range of genetic modifications including gene knockouts and gene knockings. Despite the apparent successes of the technology, the efficiency of development to live offspring has remained low and developmental abnormalities still occur. The objectives of this paper are to review some of the successes and failures of the nuclear transfer procedure since the production of Dolly. In particular, we will review the major steps in the procedure and discuss studies from our laboratory and others which have modified the procedure in ways which may impact on development.
自多利诞生至今已有8年，多利是第一只通过核移植利用从成年动物建立的供体细胞群培育出的动物。在此期间，核移植技术已成功应用于一系列哺乳动物物种，使用源自胎儿和成年组织的大量供体细胞类型来繁育后代。此外，当与供体细胞的基因操作相结合时，已培育出具有一系列基因修饰（包括基因敲除和基因敲入）的转基因后代。尽管这项技术取得了明显成功，但发育成存活后代的效率仍然很低，发育异常情况仍然会出现。本文的目的是回顾自多利诞生以来核移植程序的一些成功与失败之处。特别是，我们将回顾该程序的主要步骤，并讨论我们实验室和其他实验室的研究，这些研究以可能影响发育的方式对该程序进行了改进。
