Amin Mazyar, Kunkel Amber G, Le Victoria P, Wagenseil Jessica E
Department of Biomedical Engineering, St. Louis University, St. Louis, MO 63103, USA.
J Biomech Eng. 2011 Jul;133(7):071007. doi: 10.1115/1.4004415.
Determining arterial mechanical properties is important for understanding the work done by the heart and how it changes with cardiovascular disease. Ex vivo tests are necessary to apply various loads to the artery and obtain data to model and predict the behavior under any load. Most ex vivo tests are performed within 24 h of dissection, so the tissue is still "alive." For large elastic arteries; however, the passive mechanical behavior is attributed mostly to the very stable proteins, elastin, and collagen. If the testing equipment fails, is in use, or is located at another facility, it would be useful to store the vessels and postpone the tests until the equipment is available. The goal of this study is to determine the effects of storage time on the mechanical behavior of the common carotid artery from adult mice. Each artery was tested after storage for 1-28 days in physiologic saline at 4°C. There were no significant effects of storage time on the arterial diameter or force at each pressure, but there were significant effects on the stretch ratio and stress at each pressure. The significant effects on the stretch ratio and stress were due to decreases in the unloaded dimensions with storage time, when measured from cut arterial rings. When the unloaded dimensions were measured instead from histology sections, there were no significant changes with storage time. We conclude that histology sections yield a more consistent measurement of the unloaded dimensions and that there are no significant changes in the mechanical behavior of mouse carotid artery with storage up to 28 days.
确定动脉的力学特性对于理解心脏所做的功以及其如何随心血管疾病而变化至关重要。体外测试对于向动脉施加各种负荷并获取数据以建模和预测在任何负荷下的行为是必要的。大多数体外测试在解剖后24小时内进行，因此组织仍“存活”。然而，对于大弹性动脉，其被动力学行为主要归因于非常稳定的蛋白质，即弹性蛋白和胶原蛋白。如果测试设备出现故障、正在使用或位于另一个设施中，储存血管并将测试推迟到设备可用时将是有用的。本研究的目的是确定储存时间对成年小鼠颈总动脉力学行为的影响。每条动脉在4°C生理盐水中储存1 - 28天后进行测试。储存时间对每个压力下的动脉直径或力没有显著影响，但对每个压力下的拉伸比和应力有显著影响。对拉伸比和应力的显著影响是由于从切割的动脉环测量时，无负荷尺寸随储存时间减小。当从组织学切片测量无负荷尺寸时，储存时间没有显著变化。我们得出结论，组织学切片能更一致地测量无负荷尺寸，并且储存长达28天的小鼠颈动脉力学行为没有显著变化。
