针对衣原体外膜蛋白2的体液免疫反应分析。
Analysis of the humoral immune response to Chlamydia outer membrane protein 2.
作者信息
Mygind P, Christiansen G, Persson K, Birkelund S
机构信息
Department of Medical Microbiology and Immunology, University of Aarhus, Denmark.
出版信息
Clin Diagn Lab Immunol. 1998 May;5(3):313-8. doi: 10.1128/CDLI.5.3.313-318.1998.
Abstract
The humoral immune response to Chlamydia outer membrane protein 2 (Omp2) was studied. Omp2 is a highly genus-conserved structural protein of all Chlamydia species, containing a variable N-terminal fragment. To analyze where the immunogenic parts were localized, seven highly purified truncated fusion proteins constituting different regions of the protein were produced (Chlamydia pneumoniae-Omp2aa23-aa93, Chlamydia psittaci-Omp2aa23-aa94, and Chlamydia trachomatis-Omp2aa23-aa84, aa87-aa547, aa23-aa182, aa167-aa434, aa420-aa547). By an enzyme-linked immunosorbent assay with serologically defined patient sera, Omp2 was found to be a major immunogen of both C. pneumoniae and C. trachomatis infections (P < 0.0001). The humoral immune responses were not confined to any particular region of the Omp2 protein, and no species-specific anti-Omp2 immunoglobulins were detected.
摘要
研究了针对衣原体外膜蛋白2（Omp2）的体液免疫反应。Omp2是所有衣原体物种高度保守的结构蛋白，含有可变的N端片段。为了分析免疫原性部分的定位，制备了七种高度纯化的截短融合蛋白，它们构成了该蛋白的不同区域（肺炎衣原体-Omp2aa23-aa93、鹦鹉热衣原体-Omp2aa23-aa94、沙眼衣原体-Omp2aa23-aa84、aa87-aa547、aa23-aa182、aa167-aa434、aa420-aa547）。通过酶联免疫吸附测定法和血清学明确的患者血清，发现Omp2是肺炎衣原体和沙眼衣原体感染的主要免疫原（P < 0.0001）。体液免疫反应并不局限于Omp2蛋白的任何特定区域，未检测到物种特异性抗Omp2免疫球蛋白。
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