Halme S, Saikku P, Surcel H M
National Public Health Institute, Oulu, Finland.
Scand J Immunol. 1997 Apr;45(4):378-84. doi: 10.1046/j.1365-3083.1997.d01-413.x.
Chlamydia pneumoniae infection is followed by the development of antigen-specific cell-mediated immunity (CMI), which is detectable as a positive lymphocyte proliferation (LP) response to C. pneumoniae elementary body (EB) antigen, but the proteins inducing the T cell activation are not known. In the present work the authors used human T lymphocyte clones (TLC) raised against C. pneumoniae EB antigen to characterize C. pneumoniae proteins as T cell-stimulating antigens. A total of 55% of the TLC established recognized antigenic determinants only on C. pneumoniae species, while the rest of the TLC proliferated to both C. pneumoniae and C. trachomatis EB. The antigen specificity of the TLC was further analysed by stimulating with SDS-PAGE fractionated C. pneumoniae EB proteins. Chlamydia pneumoniae species-specific antigens were found in the molecular weight ranges 92-98, 51-55, 43-46 and 31.5-33 kDa and genus-specific antigens in the ranges 12, 26 and 65-70 kDa. The 46.5-49.5 and 55-61 kDa regions contained both species-specific and genus-specific antigens. Human leucocyte antigen (HLA) restriction analysis for the TLC isolated from an HLA DR4, 15(2) heterozygous person showed the majority (81.3%) to be restricted to the HLA DR4 molecule, the rest being DR15(2)-restricted. An interesting preliminary finding was that the expression of interferon-gamma (IFN-gamma) mRNA by the TLC was predominantly associated with antigen recognition in the context of the HLA DR4 molecule, while interleukin-4 (IL-4) production was linked to antigen recognition in the context of the HLA DR15(2) molecule.
肺炎衣原体感染后会产生抗原特异性细胞介导免疫（CMI），这种免疫可通过对肺炎衣原体原体（EB）抗原的阳性淋巴细胞增殖（LP）反应检测到，但诱导T细胞活化的蛋白质尚不清楚。在本研究中，作者使用针对肺炎衣原体EB抗原产生的人T淋巴细胞克隆（TLC）来鉴定肺炎衣原体蛋白作为T细胞刺激抗原。总共55%的已建立的TLC仅识别肺炎衣原体种的抗原决定簇，而其余的TLC对肺炎衣原体和沙眼衣原体EB均有增殖反应。通过用SDS-PAGE分级分离的肺炎衣原体EB蛋白刺激，进一步分析了TLC的抗原特异性。在分子量范围为92 - 98、51 - 55、43 - 46和31.5 - 33 kDa处发现了肺炎衣原体种特异性抗原，在12、26和65 - 70 kDa范围内发现了属特异性抗原。在46.5 - 49.5和55 - 61 kDa区域同时含有种特异性和属特异性抗原。对从一名HLA DR4、15(2)杂合子个体分离的TLC进行人类白细胞抗原（HLA）限制性分析，结果显示大多数（81.3%）受限于HLA DR4分子，其余的受DR15(2)限制。一个有趣的初步发现是，TLC产生的干扰素-γ（IFN-γ）mRNA的表达主要与HLA DR4分子背景下的抗原识别相关，而白细胞介素-4（IL-4）的产生则与HLA DR15(2)分子背景下的抗原识别有关。
