Shipulina O Iu, Shakhgil'dian V I, Shipulin G A, Kravchenko A V, Serebrovskaia L V, Pokrovskiĭ V V
Vopr Virusol. 1998 Mar-Apr;43(2):91-5.
The authors propose a simple and standard approach to semi-quantitative detection of cytomegalovirus (CMV) DNA in blood leukocytes by the polymerase chain reaction. The method was used in early diagnosis and treatment of manifest CMV infection in HIV-infected patients. High titers of CMV DNA correlated with the manifestation of CMV disease and progressive loss of host immune function. Clinical significance of difference in DNA titers was revealed. The proposed method can be used to assess the efficacy of specific anti-CMV therapy.
作者们提出了一种通过聚合酶链反应对血液白细胞中巨细胞病毒（CMV）DNA进行半定量检测的简单标准方法。该方法用于HIV感染患者显性CMV感染的早期诊断和治疗。CMV DNA的高滴度与CMV疾病的表现及宿主免疫功能的进行性丧失相关。揭示了DNA滴度差异的临床意义。所提出的方法可用于评估特异性抗CMV治疗的疗效。
