Frassinetti S, Setti L, Corti A, Farrinelli P, Montevecchi P, Vallini G
National Research Council (CNR), Soil Microbiology Center, Pisa, Italy.
Can J Microbiol. 1998 Mar;44(3):289-97. doi: 10.1139/w97-155.
Rhizobium meliloti Orange 1 was isolated from aerobic sediments of a drainage ditch receiving oil refinery leakage. This bacterium has been shown to be capable of growing on dibenzothiophene as the sole carbon and energy source. This strain can also efficaciously nodulate alfalfa plants. In cultures with dibenzothiophene, Orange 1 produces six degradation intermediates. By means of analyses with UV-visible and GC-MS spectrometry, as well as nuclear magnetic resonance spectroscopy, three of these products were identified as 3-hydroxy-2-formyl-benzothiophene (product A), benzothienopyran-2-one (product B'), and dibenzothiophene-5-oxide (product D). This suggests that R. meliloti Orange 1 metabolizes dibenzothiophene via oxidative cleavage of the aromatic ring with a mechanism analogous to that described for naphthalene degradation.
苜蓿中华根瘤菌Orange 1是从接收炼油厂泄漏物的排水沟的好氧沉积物中分离出来的。这种细菌已被证明能够以二苯并噻吩作为唯一的碳源和能源生长。该菌株还能有效地使苜蓿植物结瘤。在含有二苯并噻吩的培养物中，Orange 1产生六种降解中间体。通过紫外可见光谱、气相色谱-质谱联用以及核磁共振光谱分析，其中三种产物被鉴定为3-羟基-2-甲酰基苯并噻吩（产物A）、苯并噻吩并吡喃-2-酮（产物B'）和二苯并噻吩-5-氧化物（产物D）。这表明苜蓿中华根瘤菌Orange 1通过芳香环的氧化裂解代谢二苯并噻吩，其机制类似于萘降解中所描述的机制。
