Ramos F, Castilho M C, Silveira M I
Universidade de Coimbra, Faculdade de Farmácia, Laboratório de Bromatologia e Nutrição, Portugal.
J AOAC Int. 1998 May-Jun;81(3):544-8.
Between January 1, 1991, and December 31, 1993, bovine urine was collected for analysis of beta 2-adrenergic agonist residues. A multiresidue method was developed that was capable of detecting clenbuterol, clenpenterol, clenproperol, mabuterol, mapenterol, bromobuterol, tulobuterol, and salbutamol through enzyme-linked immunosorbent assay, liquid chromatography (LC) with photodiode array detection, and gas chromatography with mass detection in electron impact mode with selective-ion monitoring. Among 1031 samples, 24 contained clenbuterol and 1 contained salbutamol. The results confirm that clenbuterol is the beta 2-adrenergic agonist most used as bovine growth promoter in Portugal.
在1991年1月1日至1993年12月31日期间，收集牛尿液以分析β2-肾上腺素能激动剂残留量。开发了一种多残留方法，该方法能够通过酶联免疫吸附测定、带光电二极管阵列检测的液相色谱（LC）以及在电子轰击模式下采用选择性离子监测的带质谱检测的气相色谱来检测克伦特罗、氯丙那林、克喘素、马布特罗、马喷特罗、溴布特罗、妥布特罗和沙丁胺醇。在1031份样本中，24份含有克伦特罗，1份含有沙丁胺醇。结果证实，克伦特罗是葡萄牙最常被用作牛生长促进剂的β2-肾上腺素能激动剂。
